F54661

antibody from NSJ Bioreagents

Targeting: CBLIF GIF, IF, IFMH, INF, TCN3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54661

Provider

NSJ Bioreagents

Product name

Gastric Intrinsic Factor Antibody / GIF / CBLIF

Antibody type

Polyclonal

Description

This highly specific Gastric Intrinsic Factor antibody is suitable for use in Flow cytometry/Immunofluorescence/Immunohistochemistry/Western blot applications with human samples.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the Gastric Intrinsic Factor antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human K562 cell lysate with Gastric Intrinsic Factor antibody. Predicted molecular weight ~45 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of human HEK293 cells with Gastric Intrinsic Factor antibody (green) and DAPI nuclear stain (blue).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human stomach tissue with Gastric Intrinsic Factor antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human K562 cells with Gastric Intrinsic Factor antibody; Blue=isotype control, Green= Gastric Intrinsic Factor antibody.