Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF4569 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat Casein Kinase 1 alpha Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects endogenous human, mouse, and rat CK1 alpha in Western blots. In Western blots, this antibody does not cross-react with recombinant human CK1 delta or CK1 gamma ; reactivity with CK1 epsilon is unknown.
- Reactivity
- Human, Mouse, Rat
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
NP_001020276
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references CD82 inhibits canonical Wnt signalling by controlling the cellular distribution of β-catenin in carcinoma cells.
Chigita S, Sugiura T, Abe M, Kobayashi Y, Shimoda M, Onoda M, Shirasuna K
International journal of oncology 2012 Dec;41(6):2021-8
International journal of oncology 2012 Dec;41(6):2021-8
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Rat Casein Kinase 1 alpha by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, NRK rat normal kidney cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Casein Kinase 1 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4569) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Casein Kinase 1 alpha at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.