PA5-22242

antibody from Invitrogen Antibodies

Targeting: HHIP FLJ20992, HIP

Antibody data

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Validation data

Product number: PA5-22242 - Provider product page
Provider: Invitrogen Antibodies
Product name: HHIP Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: mouse brain. Predicted reactivity: Mouse (99%), Rat (98%), Xenopus laevis (83%), Rhesus Monkey (100%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Chicken/Avian
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1.34 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

HHIP protein structure - PA5-22242 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of HHIP using 50 µg of mouse brain lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a HHIP polyclonal antibody (Product # PA5-22242) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of HHIP using 30 µg of A431 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a HHIP polyclonal antibody (Product # PA5-22242) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Internal HHIP in Mouse tissue extracts (50 µg). Samples was separated by 7.5% SDS-PAGE and the membrane was probed with HHIP, Internal Polyclonal antibody (Product # PA5-22242) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of HHIP was performed by separating 50 µg of mouse tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a HHIP Polyclonal Antibody (Product # PA5-22242) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using HHIP Polyclonal Antibody (Product # PA5-22242). Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HHIP Polyclonal Antibody (Product # PA5-22242) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.