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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- PA5-29529 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HHIP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1. Predicted reactivity: Mouse (97%), Rat (96%), Rhesus Monkey (99%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.89 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using HHIP Polyclonal Antibody (Product # PA5-29529). Sample (30 µg of whole cell lysate). Lane A: NT2D1 . 7.5% SDS PAGE. HHIP Polyclonal Antibody (Product # PA5-29529) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using HHIP Polyclonal Antibody (Product # PA5-29529). Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HHIP Polyclonal Antibody (Product # PA5-29529) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
