AF1049

antibody from R&D Systems

Targeting: CTSA GSL, PPGB

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF1049

Provider

R&D Systems

Product name

Human Cathepsin A/Lysosomal Carboxypeptidase A Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects both pro and active forms of human and mouse Cathepsin A/Lysosomal Carboxypeptidase A in direct ELISAs and Western blots. In Western blots, it recognizes all forms of recombinant human Cathepsin A: single chain (55 kDa), heavy chain (32 kDa), and light chain (20 kDa). Also in Western blots, less than 1% cross-reactivity with recombinant human (rh) Cathepsin B, rhCathepsin C, rhCathepsin D, rhCathepsin L, rhCathepsin L2/V, rhCathepsin O, rhCathepsin S and rhCathepsin X/Z/P is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

P10619


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Cathepsin A is expressed in primary human antigen-presenting cells.

Reich M, Spindler KD, Burret M, Kalbacher H, Boehm BO, Burster T
Immunology letters 2010 Feb 16;128(2):143-7

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Cathepsin A/Lysosomal Carboxypeptidase A in Human Prostate. Cathepsin A/Lysosomal Carboxypeptidase A was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human Cathepsin A/Lysosomal Carboxypeptidase A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1049) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.