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Western blot
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- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00006947-M12 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00006947-M12, RRID:AB_1147217
- Product name
- Mouse Monoclonal TCN1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. TCN1 - transcobalamin I (vitamin B12 binding protein, R binder family) (3F10)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TCN1 Antibody (3F10) [H00006947-M12] - Analysis of TCN1 expression in transfected 293T cell line by TCN1 monoclonal antibody (M12), clone 3F10. Lane 1: TCN1 transfected lysatE (48.2 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TCN1 Antibody (3F10) [H00006947-M12] - Analysis of TCN1 transfected lysate using anti-TCN1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TCN1 rabbit polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: TCN1 Antibody (3F10) [H00006947-M12] - Detection limit for recombinant GST tagged TCN1 is 0.1 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TCN1 Antibody (3F10) [H00006947-M12] - Analysis of monoclonal antibody to TCN1 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml
