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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- MA5-56111 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CINP Monoclonal Antibody (K70038_12G9)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Sequence of this protein is as follows: MEAKTLGTVT PRKPVLSVSA RKIKDNAADW HNLILKWETL NDAGFTTANN IANLKISLLN KDKIELDSSS PASKENEEKV CLEYNEELEK LCEELQATLD GLTKIQVKME KLSSTTKGIC ELENYHYGEE SKRPPLFHTW PTTHFYEVSH KLLEMYRKEL LLKRTVAKEL AHTGDPDLTL SYLSMWLHQP YVESDSRLHL ESMLLETGHR AL
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- K70038_12G9
- Vial size
- 200 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CINP in 15 µg of Jurkat lysate. Sample was run on 6-18% SDS-PAGE under reducing conditions, blotted onto nitrocellulose membrane, and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. CINP band was visualized using ECL Western Blotting Substrate. Incubation with primary CINP monoclonal antibody (Product # MA5-55731) at a dilution of 1 µg/mL was used.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of CINP in 200 µg of Jurkat lysate. Samples are as follows: Lane 1: Jurkat lysate, Lane 2: CINP immunoprecipitated from Jurkat lysate, Lane3: The same as Lane 2 but KT82 was used as IgG isotype control antibody. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE, blotted onto nitrocellulose membrane, and peroxidase conjugated goat anti-mouse IgG (Fc specific) was used as the secondary antibody. The isotype control antibody was KT82. Incubation of samples with CINP monoclonal antibody (Product # MA5-55731) at a dilution of 2.5 µg was used.
