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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [5]
- Other assay [1]
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- Product number
- PA5-42050 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FAH Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: AATICKSNFK YMYWTMLQQL THHSVNGCNL RPGDLLASGT ISGPEPENFG Sequence homology: Cow: 93%; Dog: 100%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 93%; Rat: 100%; Zebrafish: 93%
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Consumptive coagulopathy of severe yellow fever occurs independently of hepatocellular tropism and massive hepatic injury.
Bailey AL, Kang LI, de Assis Barros D'Elia Zanella LGF, Silveira CGT, Ho YL, Foquet L, Bial G, McCune BT, Duarte-Neto AN, Thomas A, Raué HP, Byrnes K, Kallas EG, Slifka MK, Diamond MS
Proceedings of the National Academy of Sciences of the United States of America 2020 Dec 22;117(51):32648-32656
Proceedings of the National Academy of Sciences of the United States of America 2020 Dec 22;117(51):32648-32656
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Jurkat cell lysate using an anti-Fumarylacetoacetase polyclonal antibody (Product # PA5-42050).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (paraffin-embedded) analysis of human liver and mouse FAH KO liver tissue using an anti-Fumarylacetoacetase polyclonal antibody (Product # PA5-42050).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (paraffin-embedded) analysis of human liver, mouse KO tissue using an anti-Fumarylacetoacetase polyclonal antibody (Product # PA5-42050).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (paraffin-embedded) analysis of human liver, mouse KO tissue using an anti-Fumarylacetoacetase polyclonal antibody (Product # PA5-42050).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of FAH was performed on fah-/- (left) and wildtype (right) mouse liver paraffin sections. To expose target proteins, antigen retrieval was performed by microwaving tissues for 8-15 minutes in 10mM sodium citrate buffer (pH 6.0). Following antigen retrieval, endogenous peroxidases were blocked with 3% hydrogen peroxide-methanol for 15 min at room temperature. Tissue slides were washed with deionized water and PBS, and then blocked in 3% BSA-PBS for 30 min at room temperature. Tissues were probed with a FAH polyclonal antibody (Product # PA5-42050) diluted 1:400 in 3% BSA-PBS overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting. Images were taken at 20X magnification. Data courtesy of Antibody Data Exchange Program.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (paraffin-embedded) analysis of human kidney tissue using an anti-Fumarylacetoacetase polyclonal antibody (Product # PA5-42050).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 3. YFV infects and destroys human and macaque hepatocytes, but not murine hepatocytes. ( A ) Low-magnification photographs of serial FFPE liver sections from a moribund hFRG mouse stained brown for FAH using IHC ( Left ) and YFV RNA using in situ hybridization (ISH) ( Right ). ( B ) ISH for YFV RNA in areas of prominent tissue damage in macaques and murine livers, counterstained with hematoxylin (Scale bars, 50 mm.)
