RQ7169

antibody from NSJ Bioreagents

Targeting: FAH

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ7169

Provider

NSJ Bioreagents

Product name

FAA Antibody / FAH / Fumarylacetoacetate hydrolase

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100 µg

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the Fumarylacetoacetate hydrolase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human HCCP, 2) human HepG2, 3) rat liver, 4) rat kidney, 5) mouse liver and 6) mouse kidney tissue lysate with Fumarylacetoacetate hydrolase antibody. Predicted molecular weight: 39-46 kDa (two isoforms).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human liver cancer tissue with Fumarylacetoacetate hydrolase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE rat liver tissue with Fumarylacetoacetate hydrolase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human PC-3 cells with Fumarylacetoacetate hydrolase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Fumarylacetoacetate hydrolase antibody.