PA5-142535

antibody from Invitrogen Antibodies

Targeting: IKZF4 Eos, ZNFN1A4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-142535

Provider

Invitrogen Antibodies

Product name

IKZF4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Additional Information: This antibody is expected to recognise reported isoforms NP_071910.3; NP_001338019.1; NP_001338020.1; NP_001338021.1. This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:2,000.

Reactivity

Human, Mouse

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of IKZF4 in Mouse Heart lysate (35 µg protein in RIPA buffer) using IKZF4 Polyclonal Antibody (Product # PA5-142535) at 1 µg/mL. Detected by chemiluminescence.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of IKZF4 in K562 nuclear lysate (C) + Peptide (D), (35 µg protein in RIPA buffer) using IKZF4 Polyclonal Antibody (Product # PA5-142535) at 2 µg/mL. Detected by chemiluminescence.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of IKZF4 in HeLa cells using IKZF4 Polyclonal Antibody (Product # PA5-142535). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of IKZF4 in U2OS cells using IKZF4 Polyclonal Antibody (Product # PA5-142535). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of IKZF4 in HeLa cells (blue line) using IKZF4 Polyclonal Antibody (Product # PA5-142535). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.