Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- F55022 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- RPL18A Antibody / 60S ribosomal protein L18a / Large ribosomal subunit protein eL20
- Antibody type
- Polyclonal
- Description
- This highly specific RPL18A antibody is suitable for use in Flow cytometry/Immunohistochemistry/Western blot applications with human and mouse samples.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 0.08 ml, 0.4 ml
- Concentration
- In 1X PBS, pH 7.4, with 0.09% sodium azide
- Storage
- Aliquot the RPL18A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human HEK293 cell lysate with RPL18A antibody. Predicted molecular weight ~21 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HeLa and 2) MOLT4 cell lysate with RPL18A antibody. Predicted molecular weight ~21 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of mouse NIH 3T3 cell lysate with RPL18A antibody. Predicted molecular weight ~21 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human brain tissue with RPL18A antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human HEK293 cells with RPL18A antibody; Blue=isotype control, Green= RPL18A antibody.