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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- MA5-50059 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PIN4 Recombinant Rabbit Monoclonal Antibody (14A7)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 14A7
- Vial size
- 100 µL
- Concentration
- 2 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PIN4 in U251 whole cell lysate, MCF-7 whole cell lysate, HEK293 whole cell lysate, HeLa whole cell lysate, Jurkat whole cell lysate, PC3 whole cell lysate using a dilution of 1:1,000. Samples were incubated with PIN4 monoclonal antibody (Product # MA5-50059). Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 14, 17, 15 kDa. Observed band size: 12-17 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of MCF-7 cell with PIN4 monoclonal antibody (Product # MA5-50059) using a dilution of 1:50 and counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 598-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis with a PIN4 monoclonal antibody (Product # MA5-50059) using a dilution of 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis with a PIN4 monoclonal antibody (Product # MA5-50059) using a dilution of 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
