PA5-118843

antibody from Invitrogen Antibodies

Targeting: RPS4X CCG2, DXS306, FLJ40595, RPS4, S4, SCAR, SCR10

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-118843

Provider

Invitrogen Antibodies

Product name

RPS4X Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RPS4X in Mouse kidney using a RPS4X polyclonal antibody (Product # PA5-118843).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RPS4X in mouse liver and mouse brain using a RPS4X polyclonal antibody (Product # PA5-118843). Lane 1:Mouse liver lysates; Lane 2: Mouse brain lysates; Lane 3: Mouse brain lysates treated with blocking peptide.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RPS4X in Rat kidney, Mouse liver,HepG2 cells, Raji cells(uv treatment) using a RPS4X polyclonal antibody (Product # PA5-118843). Lane 1:Rat kidney, blocked with antigen-specific peptides, Lane 2: Rat kidney, Lane 3: Mouse liver, Lane 4: HepG2 cells, Lane 5: Raji cells(uv treatment).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of RPS4X in HeLa cells using a RPS4X polyclonal antibody (Product # PA5-118843). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1:600, was used as secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of RPS4X in HeLa cells using a RPS4X polyclonal antibody (Product # PA5-118843). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1:600, was used as secondary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of RPS4X in Human colorectal cancer using a RPS4X polyclonal antibody (Product # PA5-118843). The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of RPS4X in Mouse brain tissue using a RPS4X polyclonal antibody (Product # PA5-118843). The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.