Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- GTX129153 - Provider product page
- Provider
- GeneTex
- Product name
- G9a antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Preprogramming therapeutic response of PI3K/mTOR dual inhibitor via the regulation of EHMT2 and p27 in pancreatic cancer.
Targeting histone methyltransferase G9a inhibits growth and Wnt signaling pathway by epigenetically regulating HP1α and APC2 gene expression in non-small cell lung cancer.
G9a governs colon cancer stem cell phenotype and chemoradioresistance through PP2A-RPA axis-mediated DNA damage response.
Tian YF, Wang HC, Luo CW, Hung WC, Lin YH, Chen TY, Li CF, Lin CY, Pan MR
American journal of cancer research 2018;8(9):1812-1822
American journal of cancer research 2018;8(9):1812-1822
Targeting histone methyltransferase G9a inhibits growth and Wnt signaling pathway by epigenetically regulating HP1α and APC2 gene expression in non-small cell lung cancer.
Zhang K, Wang J, Yang L, Yuan YC, Tong TR, Wu J, Yun X, Bonner M, Pangeni R, Liu Z, Yuchi T, Kim JY, Raz DJ
Molecular cancer 2018 Oct 22;17(1):153
Molecular cancer 2018 Oct 22;17(1):153
G9a governs colon cancer stem cell phenotype and chemoradioresistance through PP2A-RPA axis-mediated DNA damage response.
Luo CW, Wang JY, Hung WC, Peng G, Tsai YL, Chang TM, Chai CY, Lin CH, Pan MR
Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology 2017 Sep;124(3):395-402
Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology 2017 Sep;124(3):395-402
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Enhanced validation
Supportive validation
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- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with G9a antibody (GTX129153) diluted at 1:2000.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- G9a antibody detects G9a protein by western blot analysis.A. 30 £gg 293T whole cell lysate/extract B. 30 £gg A431 whole cell lysate/extract C. 30 £gg HeLa whole cell lysate/extract |D. 30 £gg HepG2 whole cell lysate/extract5 % SDS-PAGEG9a antibody (GTX129153) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- G9a antibody detects G9a protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 5% SDS-PAGE, and the membrane was blotted with G9a antibody (GTX129153) diluted at 1:3000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with G9a antibody (GTX129153) diluted at 1:2000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- G9a antibody detects G9a protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: G9a protein stained by G9a antibody (GTX129153) diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- G9a antibody immunoprecipitates G9a protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : Control with 3 £gg of pre-immune rabbit IgG B : Immunoprecipitation of G9a by 3 £gg of G9a antibody (GTX129153) 5% SDS-PAGE The immunoprecipitated G9a protein was detected by G9a antibody (GTX129153) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- G9a antibody detects G9a protein at nucleus on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. G9a antibody (GTX129153) diluted at 1:250.
Supportive validation
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- Experimental details
- ChIP was performed with HeLa chromatin extract and 5 ?g of either normal rabbit IgG or anti-G9a antibody. The precipitated DNA was detected by PCR with primer set targeting to FXN.