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LearnMA5-14880
antibody from Invitrogen Antibodies
Targeting: EHMT2
BAT8, C6orf30, Em:AF134726.3, G9A, KMT1C, NG36/G9a
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- MA5-14880 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EHMT2 Monoclonal Antibody (B.133.9)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Antibody clone number
- B.133.9
- Concentration
- 21 µg/mL
Submitted references Human EHMT2/G9a activates p53 through methylation-independent mechanism.
S-adenosyl methionine is necessary for inhibition of the methyltransferase G9a by the lysine 9 to methionine mutation on histone H3.
Rada M, Vasileva E, Lezina L, Marouco D, Antonov AV, Macip S, Melino G, Barlev NA
Oncogene 2017 Feb 16;36(7):922-932
Oncogene 2017 Feb 16;36(7):922-932
S-adenosyl methionine is necessary for inhibition of the methyltransferase G9a by the lysine 9 to methionine mutation on histone H3.
Jayaram H, Hoelper D, Jain SU, Cantone N, Lundgren SM, Poy F, Allis CD, Cummings R, Bellon S, Lewis PW
Proceedings of the National Academy of Sciences of the United States of America 2016 May 31;113(22):6182-7
Proceedings of the National Academy of Sciences of the United States of America 2016 May 31;113(22):6182-7
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on Nuclear extracts (30 µg lysate) of NIH/3T3 (Lane 1), 3T3-L1 (Lane 2), Hep G2 (Lane 3), COLO 205 (Lane 4), HeLa (Lane 5), HCT116 (Lane 6) and K-562 (Lane 7). The blot was probed with Anti-EHMT2 Monoclonal Antibody (B.133.9) (Product # MA5-14880, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 160 kDa band corresponding to EHMT2 was observed across the cell lines tested. An additional band around 60 kDa was observed in the cell lines tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of G9a/EHMT2 in extracts from HeLa and 293 cells using G9a/EHMT2 monoclonal antibody (Product # MA5-14880).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of EHMT2 was achieved by transfecting HeLa cells with EHMT2 specific siRNAs (Silencer® select Product # s21468, s21469). Western blot analysis (Fig. a) was performed using whole cell extracts from the EHMT2 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with EHMT2 Monoclonal Antibody (Product # MA5-14880, 1µg/ml) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution. Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to EHMT2.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of G9a/EHMT2 in HeLa cells using a G9a/EHMT2 monoclonal antibody (Product # MA5-14880) (green). Actin filaments are labeled with a fluorescent red phalloidin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
