Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunoprecipitation [2]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX128164 - Provider product page
- Provider
- GeneTex
- Product name
- G9a antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Comparison of the neuropsychological mechanisms of 2,6-diisopropylphenol and N-methyl-D-aspartate receptor antagonist against electroconvulsive therapy-induced learning and memory impairment in depressed rats.
Liu G, Liu C, Zhang XN
Molecular medicine reports 2015 Sep;12(3):3297-3308
Molecular medicine reports 2015 Sep;12(3):3297-3308
No comments: Submit comment
Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with G9a antibody (GTX128164) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- G9a antibody detects EHMT2 protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extractB. 30 ?g A431 whole cell lysate/extractC. 30 ?g HeLa whole cell lysate/extractD. 30 ?g HepG2 whole cell lysate/extractE. 30 ?g A375 whole cell lysate/extract5% SDS-PAGEG9a antibody (GTX128164) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with G9a antibody (GTX128164) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 £gg) were separated by 5% SDS-PAGE, and the membrane was blotted with G9a antibody (GTX128164) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- G9a antibody detects EHMT2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 5 min.Green: EHMT2 protein stained by G9a antibody (GTX128164) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- EHMT2 antibody immunoprecipitates EHMT2 protein in IP experiments. IP Sample: 2 mg HeLa whole cell lysate/extract A. 40 £gg HeLa whole cell lysate/extract B. Control with 2 £gg of preimmune rabbit IgG C. Immunoprecipitation of EHMT2 protein by 2 £gg of EHMT2 antibody (GTX128164) 5% SDS-PAGE The immunoprecipitated EHMT2 protein was detected by EHMT2 antibody (GTX128164) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of G9a protein from HeLa whole cell extracts using 5 £gg of G9a antibody (GTX128164).Western blot analysis was performed using G9a antibody (GTX128164).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- G9a antibody detects EHMT2 protein at on HeLa xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HeLa xenograft. G9a antibody (GTX128164) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- G9a antibody detects G9a protein at nucleus in human rectal carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human rectal carcinoma. G9a antibody (GTX128164) diluted at 1:250.
