NB100-40825

antibody from Novus Biologicals

Targeting: EHMT2 BAT8, C6orf30, Em:AF134726.3, G9A, KMT1C, NG36/G9a

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-40825

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-40825, RRID:AB_2262142

Product name

Rabbit Polyclonal G9a/EHMT2 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

References

Submitted references

Chemoproteomics profiling of HDAC inhibitors reveals selective targeting of HDAC complexes.

Bantscheff M, Hopf C, Savitski MM, Dittmann A, Grandi P, Michon AM, Schlegl J, Abraham Y, Becher I, Bergamini G, Boesche M, Delling M, Dümpelfeld B, Eberhard D, Huthmacher C, Mathieson T, Poeckel D, Reader V, Strunk K, Sweetman G, Kruse U, Neubauer G, Ramsden NG, Drewes G
Nature biotechnology 2011 Mar;29(3):255-65

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Enhanced expression of EHMT2 is involved in the proliferation of cancer cells through negative regulation of SIAH1.

Cho HS, Kelly JD, Hayami S, Toyokawa G, Takawa M, Yoshimatsu M, Tsunoda T, Field HI, Neal DE, Ponder BA, Nakamura Y, Hamamoto R
Neoplasia (New York, N.Y.) 2011 Aug;13(8):676-84

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Enhanced expression of EHMT2 is involved in the proliferation of cancer cells through negative regulation of SIAH1.

Cho HS, Kelly JD, Hayami S, Toyokawa G, Takawa M, Yoshimatsu M, Tsunoda T, Field HI, Neal DE, Ponder BA, Nakamura Y, Hamamoto R
Neoplasia (New York, N.Y.) 2011 Aug;13(8):676-84

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: G9a/EHMT2 Antibody [NB100-40825] - Detection of Human G9A/EHMT2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G9A/EHMT2 antibody NB100-40825 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: G9a/EHMT2 Antibody [NB100-40825] - Whole cell lysate (15 and 50 ug) from HeLa cells. Antibody used for WB at 1 ug/ml.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: G9a/EHMT2 Antibody [NB100-40825] - Detection of human G9A/EHMT2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G9A/EHMT2 antibody NB100-40825 (lot NB100-40825-2) used for IP at 6 ug per reaction. G9A/EHMT2 was also immunoprecipitated by a previous lot of this antibody (NB100-40825-1) and another rabbit anti-G9A/EHMT2 antibody. For blotting immunoprecipitated G9A/EHMT2, NB100-40825 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: G9a/EHMT2 Antibody [NB100-40825] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-G9A/EHMT2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: G9a/EHMT2 Antibody [NB100-40825] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti- G9A/EHMT2 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB