21129-1-AP

antibody from Proteintech Group

Targeting: CEMIP HYBID, IR2155535, KIAA1199, TMEM2L

Provider product page for 21129-1-AP

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [43]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [2]

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Product number: 21129-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#21129-1-AP, RRID:AB_10695771
Product name: KIAA1199 antibody
Antibody type: Polyclonal
Description: KD/KO validated KIAA1199 antibody (Cat. #21129-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

CEMIP protein structure - 21129-1-AP shown in red.

Submitted references TREX tetramer disruption alters RNA processing necessary for corticogenesis in THOC6 Intellectual Disability Syndrome.

Werren EA, LaForce GR, Srivastava A, Perillo DR, Li S, Johnson K, Baris S, Berger B, Regan SL, Pfennig CD, de Munnik S, Pfundt R, Hebbar M, Jimenez-Heredia R, Karakoc-Aydiner E, Ozen A, Dmytrus J, Krolo A, Corning K, Prijoles EJ, Louie RJ, Lebel RR, Le TL, Amiel J, Gordon CT, Boztug K, Girisha KM, Shukla A, Bielas SL, Schaffer AE
Nature communications 2024 Feb 22;15(1):1640

Downregulation of KIAA1199 alleviated the activation, proliferation, and migration of hepatic stellate cells by the inhibition of epithelial-mesenchymal transition.

Liu J, Xia S, Han P, Zhang M, Wu J, Liao J
Open medicine (Warsaw, Poland) 2023;18(1):20230689

BMP6 knockdown enhances cardiac fibrosis in a mouse myocardial infarction model by upregulating AP-1/CEMIP expression.

Lu G, Ge Z, Chen X, Ma Y, Yuan A, Xie Y, Pu J
Clinical and translational medicine 2023 Jun;13(6):e1296

Downregulation of CEMIP enhances radiosensitivity by promoting DNA damage and apoptosis in colorectal cancer.

Weng J, Zhang Y, Liang W, Xie Y, Wang K, Xu Q, Ding Y, Li Y
Medical oncology (Northwood, London, England) 2023 Jan 6;40(2):73

Hyaluronan in articular cartilage: Analysis of hip osteoarthritis and osteonecrosis of femoral head.

Zhang J, Nishida Y, Koike H, Ito K, Zhuo L, Nishida K, Kimata K, Ikuta K, Sakai T, Urakawa H, Seki T, Imagama S
Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2023 Feb;41(2):307-315

KIAA1199 promotes oxaliplatin resistance and epithelial mesenchymal transition of colorectal cancer via protein O-GlcNAcylation.

Hua Q, Lu Y, Wang D, Da J, Peng W, Sun G, Gu K, Wang H, Zhu Y
Translational oncology 2023 Feb;28:101617

CEMIP, acting as a scaffold protein for bridging GRAF1 and MIB1, promotes colorectal cancer metastasis via activating CDC42/MAPK pathway.

Xu G, Zhao L, Hua Q, Wang L, Liu H, Lin Z, Jin M, Wang J, Zhou P, Yang K, Wu G, Yu D, Zhang D, Zhang T
Cell death & disease 2023 Feb 27;14(2):167

Development of Therapeutic Agent for Osteoarthritis via Inhibition of KIAA1199 Activity: Effect of Ipriflavone In Vivo.

Zhang J, Nishida Y, Koike H, Zhuo L, Ito K, Ikuta K, Sakai T, Imagama S
International journal of molecular sciences 2023 Aug 4;24(15)

KIAA1199 deficiency enhances skeletal stem cell differentiation to osteoblasts and promotes bone regeneration.

Chen L, Shi K, Ditzel N, Qiu W, Figeac F, Nielsen LHD, Tencerova M, Kowal JM, Ding M, Andreasen CM, Andersen TL, Kassem M
Nature communications 2023 Apr 10;14(1):2016

KIAA1199 Correlates With Tumor Microenvironment and Immune Infiltration in Lung Adenocarcinoma as a Potential Prognostic Biomarker.

Shen X, Mo X, Tan W, Mo X, Li L, Yu F, He J, Deng Z, Xing S, Chen Z, Yang J
Pathology oncology research : POR 2022;28:1610754

CEMIP Promotes Osteosarcoma Progression and Metastasis Through Activating Notch Signaling Pathway.

Cheng J, Zhang Y, Wan R, Zhou J, Wu X, Fan Q, He J, Tan W, Deng Y
Frontiers in oncology 2022;12:919108

A top-down approach to uncover the hidden ligandome of low-density lipoprotein receptor-related protein 1 in cartilage.

Yamamoto K, Scavenius C, Meschis MM, Gremida AME, Mogensen EH, Thøgersen IB, Bonelli S, Scilabra SD, Jensen A, Santamaria S, Ahnström J, Bou-Gharios G, Enghild JJ, Nagase H
Matrix biology : journal of the International Society for Matrix Biology 2022 Sep;112:190-218

Expression and regulation of recently discovered hyaluronidases, HYBID and TMEM2, in chondrocytes from knee osteoarthritic cartilage.

Shiozawa J, de Vega S, Yoshinaga C, Ji X, Negishi Y, Momoeda M, Nakamura T, Yoshida H, Kaneko H, Ishijima M, Okada Y
Scientific reports 2022 Oct 14;12(1):17242

Identification of metastasis-associated exoDEPs in colorectal cancer using label-free proteomics.

Liu X, Li N, Zhang C, Wu X, Zhang S, Dong G, Liu G
Translational oncology 2022 May;19:101389

Single-cell analysis of human basal cell carcinoma reveals novel regulators of tumor growth and the tumor microenvironment.

Guerrero-Juarez CF, Lee GH, Liu Y, Wang S, Karikomi M, Sha Y, Chow RY, Nguyen TTL, Iglesias VS, Aasi S, Drummond ML, Nie Q, Sarin K, Atwood SX
Science advances 2022 Jun 10;8(23):eabm7981

Lenvatinib induces anticancer activity in gallbladder cancer by targeting AKT.

Ye J, Qi L, Liang J, Zong K, Liu W, Li R, Feng R, Zhai W
Journal of Cancer 2021;12(12):3548-3557

Altered regulation of DPF3, a member of the SWI/SNF complexes, underlies the 14q24 renal cancer susceptibility locus.

Colli LM, Jessop L, Myers TA, Camp SY, Machiela MJ, Choi J, Cunha R, Onabajo O, Mills GC, Schmid V, Brodie SA, Delattre O, Mole DR, Purdue MP, Yu K, Brown KM, Chanock SJ
American journal of human genetics 2021 Sep 2;108(9):1590-1610

Hypoxia increases KIAA1199/CEMIP expression and enhances cell migration in pancreatic cancer.

Oba T, Sato N, Adachi Y, Amaike T, Kudo Y, Koga A, Kohi S, Hirata K
Scientific reports 2021 Sep 14;11(1):18193

Efficacy of hyaluronic acid on intervertebral disc inflammation: An in vitro study using notochordal cell lines and human disc cells.

Yamamoto T, Suzuki S, Fujii T, Mima Y, Watanabe K, Matsumoto M, Nakamura M, Fujita N
Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2021 Oct;39(10):2197-2208

Deletion of Hyaluronan-Binding Protein Involved in Hyaluronan Depolymerization (HYBID) Results in Attenuation of Osteoarthritis in Mice.

Momoeda M, de Vega S, Kaneko H, Yoshinaga C, Shimoda M, Nakamura T, Endo Y, Yoshida H, Kaneko K, Ishijima M, Okada Y
The American journal of pathology 2021 Nov;191(11):1986-1998

Overexpression of KIAA1199, a novel strong hyaluronidase, is a poor prognostic factor in patients with osteosarcoma.

Ito K, Nishida Y, Ikuta K, Urakawa H, Koike H, Sakai T, Zhang J, Shimoyama Y, Imagama S
Journal of orthopaedic surgery and research 2021 Jul 7;16(1):439

HYBID derived from tumor cells and tumor-associated macrophages contribute to the glioblastoma growth.

Tsuji S, Nakamura S, Yamada T, de Vega S, Okada Y, Inoue S, Shimazawa M, Hara H
Brain research 2021 Aug 1;1764:147490

Forced expression of KIAA1199, a novel hyaluronidase, inhibits tumorigenicity of low-grade chondrosarcoma.

Koike H, Nishida Y, Shinomura T, Zhuo L, Hamada S, Ikuta K, Ito K, Kimata K, Ushida T, Ishiguro N
Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2020 Sep;38(9):1942-1951

A novel metastatic promoter CEMIP and its downstream molecular targets and signaling pathway of cellular migration and invasion in SCLC cells based on proteome analysis.

Li L, Pan Y, Mo X, Wei T, Song J, Luo M, Huang G, Teng C, Liang K, Mao N, Yang J
Journal of cancer research and clinical oncology 2020 Oct;146(10):2519-2534

Serum KIAA1199 is an advanced-stage prognostic biomarker and metastatic oncogene in cholangiocarcinoma.

Zhai X, Wang W, Ma Y, Zeng Y, Dou D, Fan H, Song J, Yu X, Xin D, Du G, Jiang Z, Zhang H, Zhang X, Jin B
Aging 2020 Nov 10;12(23):23761-23777

Implication of HYBID (Hyaluronan-Binding Protein Involved in Hyaluronan Depolymerization) in Hyaluronan Degradation by Synovial Fibroblasts in Patients with Knee Osteoarthritis.

Shiozawa J, de Vega S, Cilek MZ, Yoshinaga C, Nakamura T, Kasamatsu S, Yoshida H, Kaneko H, Ishijima M, Kaneko K, Okada Y
The American journal of pathology 2020 May;190(5):1046-1058

Co-expression of KIAA1199 and hypoxia-inducible factor 1α is a biomarker for an unfavorable prognosis in hepatocellular carcinoma.

Wang D, Lu S, Zhang X, Huang L, Zhao H
Medicine 2020 Dec 11;99(50):e23369

Comprehensive histological investigation of age-related changes in dermal extracellular matrix and muscle fibers in the upper lip vermilion.

Gomi T, Imamura T
International journal of cosmetic science 2020 Aug;42(4):359-368

CEMIP upregulates BiP to promote breast cancer cell survival in hypoxia.

Banach A, Jiang YP, Roth E, Kuscu C, Cao J, Lin RZ
Oncotarget 2019 Jul 2;10(42):4307-4320

KIAA1199 promotes metastasis of colorectal cancer cells via microtubule destabilization regulated by a PP2A/stathmin pathway.

Zhao L, Zhang D, Shen Q, Jin M, Lin Z, Ma H, Huang S, Zhou P, Wu G, Zhang T
Oncogene 2019 Feb;38(7):935-949

CEMIP (KIAA1199) induces a fibrosis-like process in osteoarthritic chondrocytes.

Deroyer C, Charlier E, Neuville S, Malaise O, Gillet P, Kurth W, Chariot A, Malaise M, de Seny D
Cell death & disease 2019 Feb 4;10(2):103

KIAA1199 is a secreted molecule that enhances osteoblastic stem cell migration and recruitment.

Chen L, Shi K, Andersen TL, Qiu W, Kassem M
Cell death & disease 2019 Feb 12;10(2):126

KIAA1199 overexpression is associated with abnormal expression of EMT markers and is a novel independent prognostic biomarker for hepatocellular carcinoma.

Jiang Z, Zhai X, Shi B, Luo D, Jin B
OncoTargets and therapy 2018;11:8341-8348

Functional Genomic mRNA Profiling of Colorectal Adenomas: Identification and in vivo Validation of CD44 and Splice Variant CD44v6 as Molecular Imaging Targets.

Hartmans E, Orian-Rousseau V, Matzke-Ogi A, Karrenbeld A, de Groot DJ, de Jong S, van Dam GM, Fehrmann RS, Nagengast WB
Theranostics 2017;7(2):482-492

KIAA1199 promotes migration and invasion by Wnt/β-catenin pathway and MMPs mediated EMT progression and serves as a poor prognosis marker in gastric cancer.

Jia S, Qu T, Wang X, Feng M, Yang Y, Feng X, Ma R, Li W, Hu Y, Feng Y, Ji K, Li Z, Jiang W, Ji J
PloS one 2017;12(4):e0175058

Down-regulation of KIAA1199/CEMIP by miR-216a suppresses tumor invasion and metastasis in colorectal cancer.

Zhang D, Zhao L, Shen Q, Lv Q, Jin M, Ma H, Nie X, Zheng X, Huang S, Zhou P, Wu G, Zhang T
International journal of cancer 2017 May 15;140(10):2298-2309

KIAA1199 is induced by inflammation and enhances malignant phenotype in pancreatic cancer.

Kohi S, Sato N, Koga A, Matayoshi N, Hirata K
Oncotarget 2017 Mar 7;8(10):17156-17163

KIAA1199/CEMIP/HYBID overexpression predicts poor prognosis in pancreatic ductal adenocarcinoma.

Koga A, Sato N, Kohi S, Yabuki K, Cheng XB, Hisaoka M, Hirata K
Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.] 2017 Jan-Feb;17(1):115-122

Cochlear Cell Modeling Using Disease-Specific iPSCs Unveils a Degenerative Phenotype and Suggests Treatments for Congenital Progressive Hearing Loss.

Hosoya M, Fujioka M, Sone T, Okamoto S, Akamatsu W, Ukai H, Ueda HR, Ogawa K, Matsunaga T, Okano H
Cell reports 2017 Jan 3;18(1):68-81

Conditional knockdown of hyaluronidase 2 in articular cartilage stimulates osteoarthritic progression in a mice model.

Higuchi Y, Nishida Y, Kozawa E, Zhuo L, Arai E, Hamada S, Morita D, Ikuta K, Kimata K, Ushida T, Ishiguro N
Scientific reports 2017 Aug 1;7(1):7028

Distinct Expression Pattern of a Deafness Gene, KIAA1199, in a Primate Cochlea.

Hosoya M, Fujioka M, Okano H, Ogawa K
BioMed research international 2016;2016:1781894

Crohn's Disease Fibroblasts Overproduce the Novel Protein KIAA1199 to Create Proinflammatory Hyaluronan Fragments.

Soroosh A, Albeiroti S, West GA, Willard B, Fiocchi C, de la Motte CA
Cellular and molecular gastroenterology and hepatology 2016 May;2(3):358-368.e4

Functional proteomic analysis reveals the involvement of KIAA1199 in breast cancer growth, motility and invasiveness.

Jami MS, Hou J, Liu M, Varney ML, Hassan H, Dong J, Geng L, Wang J, Yu F, Huang X, Peng H, Fu K, Li Y, Singh RK, Ding SJ
BMC cancer 2014 Mar 15;14:194

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: human brain tissue were subjected to SDS PAGE followed by western blot with 21129-1-AP(KIAA1199 antibody) at dilution of 1:500
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using KIAA1199 antibody 21129-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human stomach cancer using 21129-1-AP(KIAA1199 antibody) at dilution of 1:50 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human stomach cancer using 21129-1-AP(KIAA1199 antibody) at dilution of 1:50 (under 40x lens)
Sample type: tissue