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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-27486 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PGD Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, Mouse heart, Mouse lung.
- Concentration
- 1 mg/mL
Submitted references Tick extracellular vesicles enable arthropod feeding and promote distinct outcomes of bacterial infection.
Oliva Chávez AS, Wang X, Marnin L, Archer NK, Hammond HL, Carroll EEM, Shaw DK, Tully BG, Buskirk AD, Ford SL, Butler LR, Shahi P, Morozova K, Clement CC, Lawres L, Neal AJO, Mamoun CB, Mason KL, Hobbs BE, Scoles GA, Barry EM, Sonenshine DE, Pal U, Valenzuela JG, Sztein MB, Pasetti MF, Levin ML, Kotsyfakis M, Jay SM, Huntley JF, Miller LS, Santambrogio L, Pedra JHF
Nature communications 2021 Jun 17;12(1):3696
Nature communications 2021 Jun 17;12(1):3696
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PGD in Mouse tissue extracts (50 µg). Samples was separated by 10% SDS-PAGE and the membrane was probed with PGD Polyclonal antibody (Product # PA5-27486) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PGD in Mouse tissue extracts (50 µg). Samples was separated by 10% SDS-PAGE and the membrane was probed with PGD Polyclonal antibody (Product # PA5-27486) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PGD Polyclonal Antibody (Product # PA5-27486). Sample (30 µg of whole cell lysate). Lane A: A549. 10% SDS PAGE. PGD Polyclonal Antibody (Product # PA5-27486) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PGD Polyclonal Antibody detects PGD protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with PGD Polyclonal Antibody (Product # PA5-27486) diluted at 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PGD Polyclonal Antibody detects PGD protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with PGD Polyclonal Antibody (Product # PA5-27486) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PGD in methanol-fixed A549 cells using a PGD polyclonal antibody (Product # PA5-27486) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PGD Polyclonal Antibody detects PGD protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. PGD Polyclonal Antibody (Product # PA5-27486) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
