Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [5]
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Validation data
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- Product number
- PA5-60608 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- A1CF Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.4 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of ACF in human cell line CACO-2 shows positivity in nucleus but excluded from the nucleoli. Samples were probed using an ACF Polyclonal Antibody (Product # PA5-60608).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ACF in human colon, kidney, liver and tonsil using ACF Polyclonal Antibody (Product # PA5-60608) (A) shows similar protein distribution across tissues to an independent ACF Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ACF in human tonsil using ACF Polyclonal Antibody (Product # PA5-60608) shows no positivity as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ACF in human kidney using ACF Polyclonal Antibody (Product # PA5-60608) shows moderate nuclear positivity in cells in tubules.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ACF in human colon using ACF Polyclonal Antibody (Product # PA5-60608) shows moderate nuclear/cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ACF in human liver using ACF Polyclonal Antibody (Product # PA5-60608) shows moderate nuclear and cytoplasmic positivity in hepatocytes.