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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- RQ7948 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- 4E-T Antibody / EIF4ENIF1
- Antibody type
- Polyclonal
- Description
- This highly specific 4E-T antibody is suitable for use in Western blot/Flow cytometry/Direct ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the 4E-T antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HeLa, 2) human 293T, 3) human MCF7, 4) human HepG2, 5) rat brain, 6) rat NRK, 7) mouse brain and 8) mouse NIH 3T3 cell lysate with 4E-T antibody. Expected molecular weight ~88/108 kDa (multiple isoforms but can be observed at ~140 kDa (possibly due to phosphorylation or high proline content-Ref 1).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human HL60 cells with 4E-T antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= 4E-T antibody.
