PA5-18235
antibody from Invitrogen Antibodies
Targeting: EIF4ENIF1
2610509L04Rik, 4E-T, Clast4, FLJ21601
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-18235 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- eIF4ENIF1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, canine, mouse, porcine and rat based on sequence homology.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot staining of HEK293 cell lysate using Product # PA5-18235 at a concentration of 0.25 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of Sea Urchin embryo cells stained for 4E-T/EIF4ENIF1. Cells were blocked with 0.01% BSA and stained with 4E-T/EIF4ENIF1 Polyclonal Antibody (Product # PA5-18235) at 1:25 dilution followed by a fluorophore-conjugated anti-goat IgG secondary antibody. Staining was visualized by confocal microscopy. 4E-T staining is depicted in green, staining for a proprietary antigen in red, and DAPI nuclear stain in blue. Data courtesy of the Innovators Program.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin embedded of Human Pancreas using Product # PA5-18235 at a concentration of 5 µg/mL. The tissue was processed by steamed antigen retrieval with citrate buffer pH 6 and stained with AP.