Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
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Validation data
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- Product number
- AP1086c - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP1086c, RRID:AB_2190786
- Product name
- ENT1 Antibody (Center)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Western blot analysis of lysates from human kidney, plasma, mouse kidney, plasma and rat kidney tissue lysate(from left to right), using GPX3 Antibody (Center)(Cat. #AP1086c). AP1086c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane."
- Primary Ab dilution
- 1:1000
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Western blot analysis of lysate from HL-60 cell line, using ENT1(Slc29a1) Antibody (Center)(Cat. #AP1086c). AP1086c was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug."
- Primary Ab dilution
- 1:1000
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- ENT1(Slc29a1) (Center) (Cat.#AP1086c) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the ENT1 antibody detected ENT1 protein (arrow).
- Primary Ab dilution
- 1:1000