Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [2]
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Validation data
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- Product number
- RQ5887 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Gasdermin D Antibody / GSDMD
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Storage
- After reconstitution, the Gasdermin D antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) SW620 and 2) Jurkat lysate with Gasdermin D antibody. Predicted molecular weight ~53 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human rectal cancer with Gasdermin D antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human U-87 MG cells with Gasdermin D antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Gasdermin D antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human ThP-1 cells with Gasdermin D antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Gasdermin D antibody.