NBP2-37755

antibody from Novus Biologicals

Targeting: MAST3 KIAA0561

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

NBP2-37755

Provider

Novus Biologicals

Product name

Rabbit Polyclonal MAST3 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: MAST3 Antibody [NBP2-37755] - Detection of Human MAST3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MAST3 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: MAST3 Antibody [NBP2-37755] - Detection of Human MAST3 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MAST3 antibody used for IP at 6 ug per reaction. MAST3 was also immunoprecipitated by rabbit anti-MAST3 antibody. For blotting immunoprecipitated MAST3, NBP2-37755 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.