10594-1-AP

antibody from Proteintech Group

Targeting: PEX14

Provider product page for 10594-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [57]
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Validations
Western blot [1]

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Product number: 10594-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#10594-1-AP, RRID:AB_2252194
Product name: PEX14 antibody
Antibody type: Polyclonal
Description: PEX14 antibody (Cat. #10594-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat, monkey and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.
Reactivity: Human, Mouse, Rat, Simian
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

PEX14 protein structure - 10594-1-AP shown in red.

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Proteomic analysis reveals that cigarette smoke exposure diminishes ovarian reserve in mice by disrupting the CREB1-mediated ovarian granulosa cell proliferation-apoptosis balance.

Xu M, Li F, Xu X, Hu N, Miao J, Zhao Y, Ji S, Wang Y, Wang L
Ecotoxicology and environmental safety 2024 Feb;271:115989

Overexpression of PEX14 results in mistargeting to mitochondria, accompanied by organelle fragmentation and clustering in human embryonic kidney cells.

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Biochimica et biophysica acta. Molecular cell research 2024 Aug;1871(6):119754

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Hepatitis C virus alters the morphology and function of peroxisomes.

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Nature communications 2023 Sep 19;14(1):5804

Transmembrane protein 135 regulates lipid homeostasis through its role in peroxisomal DHA metabolism.

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TTBK2 mutations associated with spinocerebellar ataxia type 11 disrupt peroxisome dynamics and ciliary localization of SHH signaling proteins.

Muñoz-Estrada J, Nguyen AV, Goetz SC
bioRxiv : the preprint server for biology 2023 Feb 1;

Germ cell-specific deletion of Pex3 reveals essential roles of PEX3-dependent peroxisomes in spermiogenesis.

Yao Y, Shi B, Zhang X, Wang X, Li S, Yao Y, Guo Y, Chen D, Wang B, Yuan Y, Sha J, Guo X
Journal of biomedical research 2023 Dec 8;38(1):24-36

The Key Role of Peroxisomes in Follicular Growth, Oocyte Maturation, Ovulation, and Steroid Biosynthesis.

Wang S, Yang H, Fu Y, Teng X, Wang C, Xu W
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TMEM63C mutations cause mitochondrial morphology defects and underlie hereditary spastic paraplegia.

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Brain : a journal of neurology 2022 Sep 14;145(9):3095-3107

ATP13A1 prevents ERAD of folding-competent mislocalized and misoriented proteins.

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Molecular cell 2022 Nov 17;82(22):4277-4289.e10

An AMPKα2-specific phospho-switch controls lysosomal targeting for activation.

Morrison KR, Smiles WJ, Ling NXY, Hoque A, Shea G, Ngoei KRW, Yu D, Murray-Segal L, Scott JW, Galic S, Kemp BE, Petersen J, Oakhill JS
Cell reports 2022 Feb 15;38(7):110365

Mitochondrial remodeling and ischemic protection by G protein-coupled receptor 35 agonists.

Wyant GA, Yu W, Doulamis IP, Nomoto RS, Saeed MY, Duignan T, McCully JD, Kaelin WG Jr
Science (New York, N.Y.) 2022 Aug 5;377(6606):621-629

Proteomic Identification of Coxiella burnetii Effector Proteins Targeted to the Host Cell Mitochondria During Infection.

Fielden LF, Scott NE, Palmer CS, Khoo CA, Newton HJ, Stojanovski D
Molecular & cellular proteomics : MCP 2021;20:100005

Peroxisomes contribute to intracellular calcium dynamics in cardiomyocytes and non-excitable cells.

Sargsyan Y, Bickmeyer U, Gibhardt CS, Streckfuss-Bömeke K, Bogeski I, Thoms S
Life science alliance 2021 Sep;4(9)

HIV-1 Env induces pexophagy and an oxidative stress leading to uninfected CD4(+) T cell death.

Daussy CF, Galais M, Pradel B, Robert-Hebmann V, Sagnier S, Pattingre S, Biard-Piechaczyk M, Espert L
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Labeling and measuring stressed mitochondria using a PINK1-based ratiometric fluorescent sensor.

Uesugi R, Ishii S, Matsuura A, Itakura E
The Journal of biological chemistry 2021 Nov;297(5):101279

Peroxisomal-derived ether phospholipids link nucleotides to respirasome assembly.

Bennett CF, O'Malley KE, Perry EA, Balsa E, Latorre-Muro P, Riley CL, Luo C, Jedrychowski M, Gygi SP, Puigserver P
Nature chemical biology 2021 Jun;17(6):703-710

Limited survival and impaired hepatic fasting metabolism in mice with constitutive Rag GTPase signaling.

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Nature communications 2021 Jun 16;12(1):3660

USP19 promotes hypoxia-induced mitochondrial division via FUNDC1 at ER-mitochondria contact sites.

Chai P, Cheng Y, Hou C, Yin L, Zhang D, Hu Y, Chen Q, Zheng P, Teng J, Chen J
The Journal of cell biology 2021 Jul 5;220(7)

Establishment and analysis of conditional Rab1- and Rab5-knockout cells using the auxin-inducible degron system.

Hatoyama Y, Homma Y, Hiragi S, Fukuda M
Journal of cell science 2021 Dec 15;134(24)

An engineered transcriptional reporter of protein localization identifies regulators of mitochondrial and ER membrane protein trafficking in high-throughput CRISPRi screens.

Coukos R, Yao D, Sanchez MI, Strand ET, Olive ME, Udeshi ND, Weissman JS, Carr SA, Bassik MC, Ting AY
eLife 2021 Aug 20;10

A Network of Macrophages Supports Mitochondrial Homeostasis in the Heart.

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Drp1 Tubulates the ER in a GTPase-Independent Manner.

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Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division.

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Cell death & disease 2020 Dec 8;11(12):1042

Peroxisomes control mitochondrial dynamics and the mitochondrion-dependent apoptosis pathway.

Tanaka H, Okazaki T, Aoyama S, Yokota M, Koike M, Okada Y, Fujiki Y, Gotoh Y
Journal of cell science 2019 May 31;132(11)

Peroxisome turnover and diurnal modulation of antioxidant activity in retinal pigment epithelia utilizes microtubule-associated protein 1 light chain 3B (LC3B).

Daniele LL, Caughey J, Volland S, Sharp RC, Dhingra A, Williams DS, Philp NJ, Boesze-Battaglia K
American journal of physiology. Cell physiology 2019 Dec 1;317(6):C1194-C1204

F-actin patches associated with glutamatergic synapses control positioning of dendritic lysosomes.

van Bommel B, Konietzny A, Kobler O, Bär J, Mikhaylova M
The EMBO journal 2019 Aug 1;38(15):e101183

In Vivo Deletion of β-Cell Drp1 Impairs Insulin Secretion Without Affecting Islet Oxygen Consumption.

Hennings TG, Chopra DG, DeLeon ER, VanDeusen HR, Sesaki H, Merrins MJ, Ku GM
Endocrinology 2018 Sep 1;159(9):3245-3256

Super-resolution imaging reveals the sub-diffraction phenotype of Zellweger Syndrome ghosts and wild-type peroxisomes.

Soliman K, Göttfert F, Rosewich H, Thoms S, Gärtner J
Scientific reports 2018 May 17;8(1):7809

Autophagy defects and related genetic variations in renal cell carcinoma with eosinophilic cytoplasmic inclusions.

Yu Z, Ma J, Li X, Liu Y, Li M, Wang L, Zhao M, He H, Zhang Y, Rao Q, Zhao D, Wang Y, Fan L, Li P, Liu Y, Liu F, Zhang F, Ye J, Yan Q, Guo S, Wang Z
Scientific reports 2018 Jul 2;8(1):9972

Isolation of Peroxisomes from Mouse Brain Using a Continuous Nycodenz Gradient: A Comparison to the Isolation of Liver and Kidney Peroxisomes.

Schönenberger MJ, Kovacs WJ
Methods in molecular biology (Clifton, N.J.) 2017;1595:13-26

Dysferlin mediates membrane tubulation and links T-tubule biogenesis to muscular dystrophy.

Hofhuis J, Bersch K, Büssenschütt R, Drzymalski M, Liebetanz D, Nikolaev VO, Wagner S, Maier LS, Gärtner J, Klinge L, Thoms S
Journal of cell science 2017 Mar 1;130(5):841-852

Novel regulatory roles of Mff and Drp1 in E3 ubiquitin ligase MARCH5-dependent degradation of MiD49 and Mcl1 and control of mitochondrial dynamics.

Cherok E, Xu S, Li S, Das S, Meltzer WA, Zalzman M, Wang C, Karbowski M
Molecular biology of the cell 2017 Feb 1;28(3):396-410

The functional readthrough extension of malate dehydrogenase reveals a modification of the genetic code.

Hofhuis J, Schueren F, Nötzel C, Lingner T, Gärtner J, Jahn O, Thoms S
Open biology 2016 Nov;6(11)

Spectrum of PEX1 and PEX6 variants in Heimler syndrome.

Smith CE, Poulter JA, Levin AV, Capasso JE, Price S, Ben-Yosef T, Sharony R, Newman WG, Shore RC, Brookes SJ, Mighell AJ, Inglehearn CF
European journal of human genetics : EJHG 2016 Nov;24(11):1565-1571

A Dendritic Golgi Satellite between ERGIC and Retromer.

Mikhaylova M, Bera S, Kobler O, Frischknecht R, Kreutz MR
Cell reports 2016 Jan 12;14(2):189-99

Titration of mitochondrial fusion rescues Mff-deficient cardiomyopathy.

Chen H, Ren S, Clish C, Jain M, Mootha V, McCaffery JM, Chan DC
The Journal of cell biology 2015 Nov 23;211(4):795-805

Conserved targeting information in mammalian and fungal peroxisomal tail-anchored proteins.

Buentzel J, Vilardi F, Lotz-Havla A, Gärtner J, Thoms S
Scientific reports 2015 Dec 2;5:17420

Peroxisomal lactate dehydrogenase is generated by translational readthrough in mammals.

Schueren F, Lingner T, George R, Hofhuis J, Dickel C, Gärtner J, Thoms S
eLife 2014 Sep 23;3:e03640

Hif-2α promotes degradation of mammalian peroxisomes by selective autophagy.

Walter KM, Schönenberger MJ, Trötzmüller M, Horn M, Elsässer HP, Moser AB, Lucas MS, Schwarz T, Gerber PA, Faust PL, Moch H, Köfeler HC, Krek W, Kovacs WJ
Cell metabolism 2014 Nov 4;20(5):882-897

Peroxisomes are juxtaposed to strategic sites on mitochondria.

Cohen Y, Klug YA, Dimitrov L, Erez Z, Chuartzman SG, Elinger D, Yofe I, Soliman K, Gärtner J, Thoms S, Schekman R, Elbaz-Alon Y, Zalckvar E, Schuldiner M
Molecular bioSystems 2014 Jul;10(7):1742-8

Functional analysis of PEX13 mutation in a Zellweger syndrome spectrum patient reveals novel homooligomerization of PEX13 and its role in human peroxisome biogenesis.

Krause C, Rosewich H, Woehler A, Gärtner J
Human molecular genetics 2013 Oct 1;22(19):3844-57

Charcot-Marie-Tooth disease-associated mutants of GDAP1 dissociate its roles in peroxisomal and mitochondrial fission.

Huber N, Guimaraes S, Schrader M, Suter U, Niemann A
EMBO reports 2013 Jun;14(6):545-52

Peroxisome deficiency-induced ER stress and SREBP-2 pathway activation in the liver of newborn PEX2 knock-out mice.

Kovacs WJ, Charles KN, Walter KM, Shackelford JE, Wikander TM, Richards MJ, Fliesler SJ, Krisans SK, Faust PL
Biochimica et biophysica acta 2012 Jun;1821(6):895-907

Loss of ceramide synthase 3 causes lethal skin barrier disruption.

Jennemann R, Rabionet M, Gorgas K, Epstein S, Dalpke A, Rothermel U, Bayerle A, van der Hoeven F, Imgrund S, Kirsch J, Nickel W, Willecke K, Riezman H, Gröne HJ, Sandhoff R
Human molecular genetics 2012 Feb 1;21(3):586-608

Lymphoblastoid cell lines for diagnosis of peroxisome biogenesis disorders.

Grønborg S, Krätzner R, Rosewich H, Gärtner J
JIMD reports 2011;1:29-36

The unusual extended C-terminal helix of the peroxisomal α/β-hydrolase Lpx1 is involved in dimer contacts but dispensable for dimerization.

Thoms S, Hofhuis J, Thöing C, Gärtner J, Niemann HH
Journal of structural biology 2011 Sep;175(3):362-71

A role for myelin-associated peroxisomes in maintaining paranodal loops and axonal integrity.

Kassmann CM, Quintes S, Rietdorf J, Möbius W, Sereda MW, Nientiedt T, Saher G, Baes M, Nave KA
FEBS letters 2011 Jul 21;585(14):2205-11

Typical cMRI pattern as diagnostic clue for D-bifunctional protein deficiency without apparent biochemical abnormalities in plasma.

Grønborg S, Krätzner R, Spiegler J, Ferdinandusse S, Wanders RJ, Waterham HR, Gärtner J
American journal of medical genetics. Part A 2010 Nov;152A(11):2845-9

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: human liver tissue were subjected to SDS PAGE followed by western blot with 10594-1-AP(PEX14 antibody) at dilution of 1:500
Sample type: tissue