LS-B2838

antibody from LSBio

Targeting: CASZ1 castor, cst, FLJ20321, SRG, ZNF693

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-B2838

Provider

LSBio

Product name

IHC-plus™ CASZ1 Antibody LS-B2838

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human, Mouse, Rat, Bovine

Host

Rabbit

Isotype

IgG

Storage

Store vial at -20°C or below prior to opening. For extended storage, aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Dilute only prior to immediate use.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-hCas5 Antibody - Western Blot. Western blot of affinity purified anti-hCas5 antibody shows detection of a band ~125 kD in size corresponding to hCas5 (arrowhead). Lane 1 contains a lysate from AS cells transfected with empty vector. Lane 2 contains lysate from AS cells transfected with hCas5 (predicted MW 125 kD). Lane 3 contains lysate from BE2 cells that have been shown to contain a high level of hCas mRNA (as tested by Northern blot). Lane 4 contains lysate from human muscle tissue that also shows high levels of hCas mRNA. Specific band reactivity is effectively competed when the antibody is pre-incubated with the immunizing peptide (data not shown). Personal Communication. Carol Thiele, NCI, Bethesda, MD.