LS-C745339

antibody from LSBio

Targeting: CASZ1 castor, cst, FLJ20321, SRG, ZNF693

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C745339

Provider

LSBio

Product name

CASZ1 Antibody LS-C745339

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human, Mouse, Drosophila

Host

Rabbit

Isotype

IgG

Storage

Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western Blot of Anti-CASZ1 Antibody. Lane 1: NBLS Cytoplasmic (20µg). Lane 2: NBLS Nuclear (3µg). Lane 3: BE2C Cytoplasmic (30µg). Lane 4: BE2C Nuclear (7µg). Lane 5: SY5Y-CASZ1b (10µg). Block: 5% Blotto/TTBS for 1 hour. Primary: Casz1 1:10,000 for 1 hour. Secondary: Goat anti-Rabbit HRP for 1 hour. 240sec exposure. Detects nuclear endogenous CASZ1a and CASZ1b; and transiently transfected CASZ1b isoform.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot using the anti-hCASZ1 antibody. This blot shows detection of endogenous and transfected human CASZ1 protein in fresh whole cell lysate (~30 µg). Protein was resolved by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1,000, incubated 1.5 hours at room temperature, and incubated with HRP-conjugated Goat Anti-Rabbit antibody for 45 min. at room temperature. Lane 1, BE2(s) cell lysate. Lane 2, BE2(N) cell lysate. Lane 3, SY5Y transfected with hCas5 (125kDa). Lane 4, SY5Y transfected with hCas11 (190kDa).

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry results of rabbit Anti-hCasz1 Antibody. Tissue: NB patient tumor. A. CASZ1 localized exclusively in the cytoplasm. B. CASZ1 localized in the cytoplasm and nucleus. Primary Antibody: Rabbit Anti-CASZ1 stained brown. Nucleus counterstained with hematoxylin (blue).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry results of rabbit Anti-hCasz1 Antibody. Tissue: NB patient tumor. A. CASZ1 localized exclusively in the cytoplasm. B. CASZ1 localized in the cytoplasm and nucleus. Primary Antibody: Rabbit Anti-CASZ1 stained brown. Nucleus counterstained with hematoxylin (blue).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry results of rabbit Anti-hCASZ1 Antibody. Tissue: NB patient tumor. A. Score 0- a rare positive nuclei. B. Score 1- (1-10% positive) equivocal/uninterpretable. C. Score 2- (10-50% positive) weak positive. D. Score 3- (>50% positive) strong positive. Primary Antibody: Rabbit Anti-CASZ1 stained brown. Nucleus counterstained with hematoxylin (blue). Localization: Nuclear.