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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AP09334PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP09334PU-N, RRID:AB_2035246
- Product name
- anti CASZ1
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding to region near the amino terminal end of human Cas5 protein
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.3 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot using affinity purified anti-hCas5 antibody shows detection of a band ~125 kDa in size corresponding to hCas5 (arrowhead). Lane 1 contains a lysate from AS cells transfected with empty vector. Lane 2 contains lysate from AS cells transfected with hCas5 (predicted MW 125 kDa). Lane 3 contains lysate from BE2 cells that have been shown to contain a high level of hCas mRNA (as tested by Northern blot). Lane 4 contains lysate from human muscle tissue that also shows high levels of hCas mRNA. Specific band reactivity is effectively competed when the antibody is pre-incubated with the immunizing peptide (data not shown).
