AP09334PU-N

antibody from Acris Antibodies GmbH

Targeting: CASZ1 castor, cst, FLJ20321, SRG, ZNF693

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

AP09334PU-N

Provider

Acris Antibodies GmbH

Proper citation

Product name

anti CASZ1

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding to region near the amino terminal end of human Cas5 protein

Reactivity

Human, Mouse, Rat, Bovine

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

1.3 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot using affinity purified anti-hCas5 antibody shows detection of a band ~125 kDa in size corresponding to hCas5 (arrowhead). Lane 1 contains a lysate from AS cells transfected with empty vector. Lane 2 contains lysate from AS cells transfected with hCas5 (predicted MW 125 kDa). Lane 3 contains lysate from BE2 cells that have been shown to contain a high level of hCas mRNA (as tested by Northern blot). Lane 4 contains lysate from human muscle tissue that also shows high levels of hCas mRNA. Specific band reactivity is effectively competed when the antibody is pre-incubated with the immunizing peptide (data not shown).