12436-1-AP

antibody from Invitrogen Antibodies

Targeting: SHC3 N-Shc, NSHC, SHCC

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12436-1-AP

Provider

Invitrogen Antibodies

Product name

SHC3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: IIANHHMRS ISFASGGDPD TTDYVAYVTK DPVNRRACHI LECCDGLAQD VIGSIGQAFE LRFKQYLQCP TKIPALHDRM QSLDEPWTEE EGDGSDHPYY NSIPSKMPPP GGFLDTRLKP RPHAPDTAQF AGKEQTYYQG RHLGDTFGED WQQTPLRQGS SDIYSTPEGK LHVAPTGEAP TYVNTQQIPP QAWPAAVSSA ESSPRKDLFD MKPFEDALKN QPLGPVLSKA ASVECISPVS PRAPDAKMLE ELQAETWYQG EMSRKEAEGL LEKDGDFLVR KSTTNPGSFV LTGMHNGQAK HLLLVDPEGT IQTKDRVFDS ISHLINHHLE SSLPIVSAGS ELCLQQPVER KQ (244-594 aa encoded by BC026314)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.33 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12436-1-AP (SHC3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12436-1-AP (SHC3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas cancer using 12436-1-AP (SHC3 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas cancer using 12436-1-AP (SHC3 antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using (Product # 12436-1-AP) (SHC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-SHC3 (IP:12436-1-AP, 4ug; Detection:12436-1-AP 1:300) with BxPC-3 cells lysate 2800ug.