Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [3]
- Other assay [1]
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Validation data
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- Product number
- 12436-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SHC3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: IIANHHMRS ISFASGGDPD TTDYVAYVTK DPVNRRACHI LECCDGLAQD VIGSIGQAFE LRFKQYLQCP TKIPALHDRM QSLDEPWTEE EGDGSDHPYY NSIPSKMPPP GGFLDTRLKP RPHAPDTAQF AGKEQTYYQG RHLGDTFGED WQQTPLRQGS SDIYSTPEGK LHVAPTGEAP TYVNTQQIPP QAWPAAVSSA ESSPRKDLFD MKPFEDALKN QPLGPVLSKA ASVECISPVS PRAPDAKMLE ELQAETWYQG EMSRKEAEGL LEKDGDFLVR KSTTNPGSFV LTGMHNGQAK HLLLVDPEGT IQTKDRVFDS ISHLINHHLE SSLPIVSAGS ELCLQQPVER KQ (244-594 aa encoded by BC026314)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.33 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12436-1-AP (SHC3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12436-1-AP (SHC3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human pancreas cancer using 12436-1-AP (SHC3 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human pancreas cancer using 12436-1-AP (SHC3 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using (Product # 12436-1-AP) (SHC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-SHC3 (IP:12436-1-AP, 4ug; Detection:12436-1-AP 1:300) with BxPC-3 cells lysate 2800ug.