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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
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- Product number
- GTX83559 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX83559, RRID:AB_10728941
- Product name
- SSB antibody [3F11]
- Antibody type
- Monoclonal
- Reactivity
- Human, Canine, Simian
- Host
- Mouse
- Storage
- For short-term storage, store at 4¢XC or aliquot into working amounts and store at -20¢XC. For long-term storage, store at -70¢XC (aliquotted). Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSB.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-anti-SSBmonoclonal antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Anti-SSB mouse monoclonal antibody (GTX83559) immunofluorescent staining of COS7 cells transiently transfected with SSB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation (IP) of SSB by using TrueMab monoclonal anti-SSB antibodies (Negative control: IP without adding anti-SSB antibody.). For each experiment, 500ul of DDK tagged SSB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-SSB antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-SSBmouse monoclonal antibody. (GTX83559, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded prostate tissue using anti-SSBmouse monoclonal antibody. (GTX83559, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded lung tissue using anti-SSBmouse monoclonal antibody. (GTX83559, Dilution 1:50)
