MA5-24921

antibody from Invitrogen Antibodies

Targeting: SSB La, La/SSB, LARP3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

MA5-24921

Provider

Invitrogen Antibodies

Product name

SSB Monoclonal Antibody (OTI9D6)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human, Canine

Host

Mouse

Isotype

IgG

Antibody clone number

OTI9D6

Vial size

100 µL

Concentration

0.63 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation was performed on HEK293T cells overexpressing SSB. Antigen-antibody complexes were formed by incubating the sample with 2 µg of SSB monoclonal antibody (Product # MA5-24921) antibody and 0.1 mg of goat anti-mouse conjugated magnetic beads overnight. The sample was washed extensively and probed with rabbit anti-DDK polyclonal antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SSB in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with SSB (Product # MA5-24921) monoclonal antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SSB in HepG2, HeLa, HT29, A549, COS7, Jurkat, MDCK, PC12, MCF7 cells using 35 µg per lane. Samples were probed with SSB (Product # MA5-24921) monoclonal antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SSB in COS7 cells. Cells were transfected with a plasmid overexpressing SSB and probed with a SSB monoclonal antibody (Product # MA5-24921).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SSB in COS7 cells. Cells were transfected with a plasmid overexpressing SSB and probed with a SSB monoclonal antibody (Product # MA5-24921).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SSB in COS7 cells. Cells were transfected with a plasmid overexpressing SSB and probed with a SSB monoclonal antibody (Product # MA5-24921).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SSB in COS7 cells. Cells were transfected with a plasmid overexpressing SSB and probed with a SSB monoclonal antibody (Product # MA5-24921).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SSB in COS7 cells. Cells were transfected with a plasmid overexpressing SSB and probed with a SSB monoclonal antibody (Product # MA5-24921).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry was performed on paraffin-embedded human kidney tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a SSB monoclonal antibody (Product # MA5-24921).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry was performed on paraffin-embedded human breast tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a SSB monoclonal antibody (Product # MA5-24921).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry was performed on paraffin-embedded human lung tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a SSB monoclonal antibody (Product # MA5-24921).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry was performed on paraffin-embedded human prostate tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a SSB monoclonal antibody (Product # MA5-24921).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry was performed on paraffin-embedded human ovary tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a SSB monoclonal antibody (Product # MA5-24921).