Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-29763 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SSB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, JC, C2C12.
- Concentration
- 0.52 mg/mL
Submitted references Endoplasmic reticulum stress causes autophagy and apoptosis leading to cellular redistribution of the autoantigens Ro/Sjögren's syndrome-related antigen A (SSA) and La/SSB in salivary gland epithelial cells.
Katsiougiannis S, Tenta R, Skopouli FN
Clinical and experimental immunology 2015 Aug;181(2):244-52
Clinical and experimental immunology 2015 Aug;181(2):244-52
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SSB Polyclonal Antibody (Product # PA5-29763). Sample (30 µg of whole cell lysate). Lane A: 293T. 10% SDS PAGE. SSB Polyclonal Antibody (Product # PA5-29763) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SSB Polyclonal Antibody (Product # PA5-29763). Sample (30 µg of whole cell lysate). A: JC. B: C2C12. 7.5% SDS PAGE. SSB Polyclonal Antibody (Product # PA5-29763) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SSB in paraformaldehyde-fixed A431 cells using a SSB polyclonal antibody (Product # PA5-29763) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using SSB (Product # PA5-29763) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SSB Polyclonal Antibody detects SSB protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. SSB Polyclonal Antibody (Product # PA5-29763) dilution: 1:1,000. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SSB Polyclonal Antibody detects SSB protein at nucleus on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. SSB Polyclonal Antibody (Product # PA5-29763) dilution: 1:1,000. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
