Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA505585 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA505585, RRID:AB_2623204
- Product name
- GBAS mouse monoclonal antibody, clone OTI1D6 (formerly 1D6)
- Antibody type
- Monoclonal
- Description
- GBAS mouse monoclonal antibody, clone OTI1D6 (formerly 1D6)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- NIPSNAP2
- Isotype
- IgG
- Antibody clone number
- OTI1D6
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBAS (RC205027, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBAS.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GBAS monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)(1:200).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-GBAS mouse monoclonal antibody (TA505585).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-GBAS mouse monoclonal antibody (TA505585) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GBAS(RC205027).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC205027 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-GBAS antibody(TA505585), and then analyzed by flow cytometry.
- Validation comment
- FC
