NBP2-22164

antibody from Novus Biologicals

Targeting: SLC6A3 DAT, DAT1

Provider product page for NBP2-22164

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [12]
Comments [0]
Validations
Western blot [2]
Immunohistochemistry [1]

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Product number: NBP2-22164 - Provider product page
Provider: Novus Biologicals
Product name: Mouse Monoclonal SLC6A3/DAT1 Antibody
Antibody type: Monoclonal
Description: Protein G purified.
Reactivity: Human, Mouse, Rat
Host: Mouse
Antigen sequence: N-terminus
Isotype: IgG
Vial size: 0.1 ml
Concentration: 1.0 mg/ml
Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

SLC6A3 protein structure - NBP2-22164 shown in red.

Submitted references Brain tyrosinase overexpression implicates age-dependent neuromelanin production in Parkinson's disease pathogenesis.

Carballo-Carbajal I, Laguna A, Romero-Giménez J, Cuadros T, Bové J, Martinez-Vicente M, Parent A, Gonzalez-Sepulveda M, Peñuelas N, Torra A, Rodríguez-Galván B, Ballabio A, Hasegawa T, Bortolozzi A, Gelpi E, Vila M
Nature communications 2019 Mar 7;10(1):973

Phosphorylation of dopamine transporter serine 7 modulates cocaine analog binding.

Moritz AE, Foster JD, Gorentla BK, Mazei-Robison MS, Yang JW, Sitte HH, Blakely RD, Vaughan RA
The Journal of biological chemistry 2013 Jan 4;288(1):20-32

Dopamine transporter phosphorylation site threonine 53 regulates substrate reuptake and amphetamine-stimulated efflux.

Foster JD, Yang JW, Moritz AE, Challasivakanaka S, Smith MA, Holy M, Wilebski K, Sitte HH, Vaughan RA
The Journal of biological chemistry 2012 Aug 24;287(35):29702-12

Palmitoylation controls dopamine transporter kinetics, degradation, and protein kinase C-dependent regulation.

Foster JD, Vaughan RA
The Journal of biological chemistry 2011 Feb 18;286(7):5175-86

Syntaxin 1A regulates dopamine transporter activity, phosphorylation and surface expression.

Cervinski MA, Foster JD, Vaughan RA
Neuroscience 2010 Oct 13;170(2):408-16

Leptin promotes dopamine transporter and tyrosine hydroxylase activity in the nucleus accumbens of Sprague-Dawley rats.

Perry ML, Leinninger GM, Chen R, Luderman KD, Yang H, Gnegy ME, Myers MG Jr, Kennedy RT
Journal of neurochemistry 2010 Aug;114(3):666-74

Dopamine and amphetamine rapidly increase dopamine transporter trafficking to the surface: live-cell imaging using total internal reflection fluorescence microscopy.

Furman CA, Chen R, Guptaroy B, Zhang M, Holz RW, Gnegy M
The Journal of neuroscience : the official journal of the Society for Neuroscience 2009 Mar 11;29(10):3328-36

Proline-directed phosphorylation of the dopamine transporter N-terminal domain.

Gorentla BK, Moritz AE, Foster JD, Vaughan RA
Biochemistry 2009 Feb 10;48(5):1067-76

C57BL/6J mice show greater amphetamine-induced locomotor activation and dopamine efflux in the striatum than 129S2/SvHsd mice.

Chen R, Zhang M, Park S, Gnegy ME
Pharmacology, biochemistry, and behavior 2007 May;87(1):158-63

Rapid delivery of the dopamine transporter to the plasmalemmal membrane upon amphetamine stimulation.

Johnson LA, Furman CA, Zhang M, Guptaroy B, Gnegy ME
Neuropharmacology 2005 Nov;49(6):750-8

Regulation of amphetamine-stimulated dopamine efflux by protein kinase C beta.

Johnson LA, Guptaroy B, Lund D, Shamban S, Gnegy ME
The Journal of biological chemistry 2005 Mar 25;280(12):10914-9

Uptake inhibitors but not substrates induce protease resistance in extracellular loop two of the dopamine transporter.

Gaffaney JD, Vaughan RA
Molecular pharmacology 2004 Mar;65(3):692-701

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: DAT1 Antibody (mAb16) [NBP2-22164] - Analysis of DAT1 expression in rat striatal membrane tissue lysate.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: SLC6A3/DAT1 Antibody (mAb16) [NBP2-22164] - Western blot analysis of DAT protein. Band shown at approx. 75 kDa. Image submitted by verified customer review.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: DAT1 Antibody (mAb16) [NBP2-22164] - IHC analysis of DAT1 in mouse brain using DAB with hematoxylin counterstain.