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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- NBP2-29533 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal SLC6A3/DAT1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specific for the ~55k glycosylated form of the DAT protein phosphorylated at Thr53. Relative mobility may vary depending on the state of glycosylation of the DAT protein. The antibody works best in lysates that have not been boiled prior to being run on an SDS-PAGE gel. Immunolabeling of the DAT band is blocked by preadsorption with the phospho-peptide used as antigen but not by the corresponding dephospho-peptide.
- Reactivity
- Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: DAT1 [p Thr53] Antibody [NBP2-29533] - Western blot of rat caudate lysate showing specific immunolabeling of the ~55k glycosylated form of the DAT protein phosphorylated at Thr53. Immunolabeling is blocked by the phospho-peptide used as antigen (peptide), but not by the corresponding dephospho-peptide (not shown).
