22524-1-AP

antibody from Proteintech Group

Targeting: SLC6A3 DAT, DAT1

Provider product page for 22524-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [41]
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Validations
Immunohistochemistry [1]

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Product number: 22524-1-AP - Provider product page
Provider: Proteintech Group
Product name: DAT antibody
Antibody type: Polyclonal
Description: DAT antibody (Cat. #22524-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

SLC6A3 protein structure - 22524-1-AP shown in red.

Submitted references Comparative analysis of uninduced and neuronally-induced human dental pulp stromal cells in a 6-OHDA model of Parkinson's disease.

Azevedo EM, Fracaro L, Hochuli AHD, Ilkiw J, Bail EL, Lisboa MO, Rodrigues LS, Barchiki F, Correa A, Capriglione LGA, Brofman PRS, Lima MMS
Cytotherapy 2024 Sep;26(9):1052-1061

Effects of latroeggtoxin-VI on dopamine and α-synuclein in PC12 cells and the implications for Parkinson's disease.

Yu D, Wang H, Zhai Y, Lei Z, Sun M, Chen S, Yin P, Wang X
Biological research 2024 Mar 16;57(1):9

Impaired Response to Mismatch Novelty in the Li(2+)-Pilocarpine Rat Model of TLE: Correlation with Hippocampal Monoaminergic Inputs.

Nascimento C, Guerreiro-Pinto V, Pawlak S, Caulino-Rocha A, Amat-Garcia L, Cunha-Reis D
Biomedicines 2024 Mar 12;12(3)

Sleep and Pain: A Role for the Anterior Cingulate Cortex, Nucleus Accumbens, and Dopamine in the Increased Pain Sensitivity Following Sleep Restriction.

Sardi NF, Pescador AC, Azevedo EM, Pochapski JA, Kukolj C, Spercoski KM, Andrade AJM, da Cunha C, Fischer L
The journal of pain 2024 Feb;25(2):331-349

Dopamine regulates colonic glial cell-derived neurotrophic factor secretion through cholinergic dependent and independent pathways.

Zhang XL, Sun Q, Quan ZS, Wu L, Liu ZM, Xia YQ, Wang QY, Zhang Y, Zhu JX
British journal of pharmacology 2024 Feb;181(3):413-428

Electroacupuncture alleviated depression-like behaviors in ventromedial prefrontal cortex of chronic unpredictable mild stress-induced rats: Increasing synaptic transmission and phosphorylating dopamine transporter.

Cai X, Wu M, Zhang Z, Liu H, Huang S, Song J, Ren S, Huang Y
CNS neuroscience & therapeutics 2023 Sep;29(9):2608-2620

Recovery of motor function is associated with rescue of glutamate biomarkers in the striatum and motor cortex following treatment with Mucuna pruriens in a murine model of Parkinsons disease.

Denne T, Winfrey LC, Moore C, Whitner C, D'Silva T, Soumyanath A, Shinto L, Hiller A, Meshul CK
Molecular and cellular neurosciences 2023 Sep;126:103883

Low-dose Cu exposure enhanced α-synuclein accumulation associates with mitochondrial impairments in mice model of Parkinson's disease.

Chen J, Gao X, Zheng C, Zhang C, Li P, He K, Liu G, Huang X, Liu J, Xie Y, Yang X
Toxicology letters 2023 Sep 15;387:14-27

Effectiveness of exercise and pramipexole in the treatment of restless leg syndrome: Implications on the dopaminergic system and PTPRD.

Morais MA, Franco B, Holanda ASS, Simino LAP, Manconi M, Torsoni A, Esteves AM
Sleep medicine 2023 Oct;110:201-211

Phosphoglycerate kinase is a central leverage point in Parkinson's Disease driven neuronal metabolic deficits.

Kokotos AC, Antoniazzi AM, Unda SR, Ko MS, Park D, Eliezer D, Kaplitt MG, Camilli P, Ryan TA
bioRxiv : the preprint server for biology 2023 Oct 10;

Socially Mediated Shift in Neural Circuits Activation Regulated by Synergistic Neuromodulatory Signaling.

Clements KN, Ahn S, Park C, Heagy FK, Miller TH, Kassai M, Issa FA
eNeuro 2023 Nov;10(11)

Crucial neuroprotective roles of the metabolite BH4 in dopaminergic neurons.

Cronin SJF, Yu W, Hale A, Licht-Mayer S, Crabtree MJ, Korecka JA, Tretiakov EO, Sealey-Cardona M, Somlyay M, Onji M, An M, Fox JD, Turnes BL, Gomez-Diaz C, da Luz Scheffer D, Cikes D, Nagy V, Weidinger A, Wolf A, Reither H, Chabloz A, Kavirayani A, Rao S, Andrews N, Latremoliere A, Costigan M, Douglas G, Freitas FC, Pifl C, Walz R, Konrat R, Mahad DJ, Koslov AV, Latini A, Isacson O, Harkany T, Hallett PJ, Bagby S, Woolf CJ, Channon KM, Je HS, Penninger JM
bioRxiv : the preprint server for biology 2023 May 8;

Dysregulation of striatal dopamine D2/D3 receptor-mediated by hypocretin induces depressive behaviors in rats.

Chen B, Xu J, Chen S, Mou T, Wang Y, Wang H, Zhang Z, Ren F, Wang Z, Jin K, Lu J
Journal of affective disorders 2023 Mar 15;325:256-263

Components of the sympathetic nervous system as targets to modulate inflammation - rheumatoid arthritis synovial fibroblasts as neuron-like cells?

Cheng X, Lowin T, Honke N, Pongratz G
Journal of inflammation (London, England) 2023 Mar 14;20(1):9

Multi-omics analysis reveals BDE47 induces depression-like behaviors in mice by interfering with the 2-arachidonoyl glycerol-associated microbiota-gut-brain axis.

Wang CQ, Su Z, Dai CG, Song JL, Qian B
Ecotoxicology and environmental safety 2023 Jul 1;259:115041

Exendin-4 and linagliptin attenuate neuroinflammation in a mouse model of Parkinson's disease.

Yu HY, Sun T, Wang Z, Li H, Xu D, An J, Wen LL, Li JY, Li W, Feng J
Neural regeneration research 2023 Aug;18(8):1818-1826

Focused Ultrasound Promotes the Delivery of Gastrodin and Enhances the Protective Effect on Dopaminergic Neurons in a Mouse Model of Parkinson's Disease.

Wang Y, Luo K, Li J, Liao Y, Liao C, Chen WS, Chen M, Ao L
Frontiers in cellular neuroscience 2022;16:884788

Fine Particulate Matter Triggers α-Synuclein Fibrillization and Parkinson-like Neurodegeneration.

Yuan X, Yang Y, Liu C, Tian Y, Xia D, Liu Z, Pan L, Xiong M, Xiong J, Meng L, Zhang Z, Ye K, Jiang H, Zhang Z
Movement disorders : official journal of the Movement Disorder Society 2022 Sep;37(9):1817-1830

Sleep Disturbance Alters Cocaine-Induced Locomotor Activity: Involvement of Striatal Neuroimmune and Dopamine Signaling.

Roodsari SK, Cheng Y, Reed KM, Wellman LL, Sanford LD, Kim WK, Guo ML
Biomedicines 2022 May 18;10(5)

Sun X, Kato H, Sato H, Han X, Hirofuji Y, Kato TA, Sakai Y, Ohga S, Fukumoto S, Masuda K
FASEB bioAdvances 2022 Jul;4(7):454-467

FMR1 deletion in rats induces hyperactivity with no changes in striatal dopamine transporter availability.

D'Elia A, Schiavi S, Manduca A, Rava A, Buzzelli V, Ascone F, Orsini T, Putti S, Soluri A, Galli F, Soluri A, Mattei M, Cicconi R, Massari R, Trezza V
Scientific reports 2022 Dec 29;12(1):22535

Reconstituting neurovascular unit with primary neural stem cells and brain microvascular endothelial cells in three-dimensional matrix.

Wang H, Yang H, Shi Y, Xiao Y, Yin Y, Jiang B, Ren H, Chen W, Xue Q, Xu X
Brain pathology (Zurich, Switzerland) 2021 Sep;31(5):e12940

Exposure to Melamine cyanuric acid in adolescent mice caused emotional disorder and behavioral disorder.

Wang S, Zhang J, Zhang S, Shi F, Feng D, Feng X
Ecotoxicology and environmental safety 2021 Mar 15;211:111938

Asparagine endopeptidase cleaves synaptojanin 1 and triggers synaptic dysfunction in Parkinson's disease.

Zou L, Zhang X, Xiong M, Meng L, Tian Y, Pan L, Yuan X, Chen G, Wang Z, Bu L, Yao Z, Zhang Z, Ye K, Zhang Z
Neurobiology of disease 2021 Jul;154:105326

The neuroprotective effects of isoquercitrin purified from apple pomace by high-speed countercurrent chromatography in the MPTP acute mouse model of Parkinson's disease.

Liu C, Wang W, Li H, Liu J, Zhang P, Cheng Y, Qin X, Hu Y, Wei Y
Food & function 2021 Jul 7;12(13):6091-6101

Iron deficiency in pregnancy: Influence on sleep, behavior, and molecular markers of adult male offspring.

Franco B, Mota DS, Daubian-Nosé P, Rodrigues NA, Simino LAP, de Fante T, Bezerra RMN, Manchado Gobatto FB, Manconi M, Torsoni AS, Esteves AM
Journal of neuroscience research 2021 Dec;99(12):3325-3338

Gait Deficits and Loss of Striatal Tyrosine Hydroxlase/Trk-B are Restored Following 7,8-Dihydroxyflavone Treatment in a Progressive MPTP Mouse Model of Parkinson's Disease.

Massaquoi MS, Liguore WA, Churchill MJ, Moore C, Melrose HL, Meshul CK
Neuroscience 2020 May 1;433:53-71

Reversing a model of Parkinson's disease with in situ converted nigral neurons.

Qian H, Kang X, Hu J, Zhang D, Liang Z, Meng F, Zhang X, Xue Y, Maimon R, Dowdy SF, Devaraj NK, Zhou Z, Mobley WC, Cleveland DW, Fu XD
Nature 2020 Jun;582(7813):550-556

PLRP2 selectively localizes synaptic membrane proteins via acyl-chain remodeling of phospholipids.

Kuge H, Miyamoto I, Yagyu KI, Honke K
Journal of lipid research 2020 Dec;61(12):1747-1763

Catalpol Exerts a Neuroprotective Effect in the MPTP Mouse Model of Parkinson's Disease.

Wang LY, Yu X, Li XX, Zhao YN, Wang CY, Wang ZY, He ZY
Frontiers in aging neuroscience 2019;11:316

Differentiation of adult human retinal pigment epithelial cells into dopaminergic-like cells in vitro and in the recipient monkey brain.

Li S, Zhang H, Wang A, Liu Y, Liu H, Yue F, Abulaiti X, Zhang C, Li L
Molecular medicine (Cambridge, Mass.) 2019 Mar 29;25(1):9

Effects of sleep disruption on stress, nigrostriatal markers, and behavior in a chronic/progressive MPTP male mouse model of parkinsonism.

Xu M, Bohlen JK, Moore C, Nipper MA, Finn DA, Jones CE, Lim MM, Meshul CK
Journal of neuroscience research 2019 Dec;97(12):1706-1719

Andrographolide alleviates Parkinsonism in MPTP-PD mice via targeting mitochondrial fission mediated by dynamin-related protein 1.

Geng J, Liu W, Gao J, Jiang C, Fan T, Sun Y, Qin ZH, Xu Q, Guo W, Gao J
British journal of pharmacology 2019 Dec;176(23):4574-4591

The loss of dopaminergic neurons in DEC1 deficient mice potentially involves the decrease of PI3K/Akt/GSK3β signaling.

Zhu Z, Yichen W, Ziheng Z, Dinghao G, Ming L, Wei L, Enfang S, Gang H, Honda H, Jian Y
Aging 2019 Dec 28;11(24):12733-12753

Glatiramer Acetate Reverses Motor Dysfunction and the Decrease in Tyrosine Hydroxylase Levels in a Mouse Model of Parkinson's Disease.

Churchill MJ, Cantu MA, Kasanga EA, Moore C, Salvatore MF, Meshul CK
Neuroscience 2019 Aug 21;414:8-27

Downregulation of DEC1 contributes to the neurotoxicity induced by MPP(+) by suppressing PI3K/Akt/GSK3β pathway.

Zhu Z, Wang YW, Ge DH, Lu M, Liu W, Xiong J, Hu G, Li XP, Yang J
CNS neuroscience & therapeutics 2017 Sep;23(9):736-747

Exercise in an animal model of Parkinson's disease: Motor recovery but not restoration of the nigrostriatal pathway.

Churchill MJ, Pflibsen L, Sconce MD, Moore C, Kim K, Meshul CK
Neuroscience 2017 Sep 17;359:224-247

Echinacoside's nigrostriatal dopaminergic protection against 6-OHDA-Induced endoplasmic reticulum stress through reducing the accumulation of Seipin.

Zhang Y, Long H, Zhou F, Zhu W, Ruan J, Zhao Y, Lu Y
Journal of cellular and molecular medicine 2017 Dec;21(12):3761-3775

Exercise intervention increases spontaneous locomotion but fails to attenuate dopaminergic system loss in a progressive MPTP model in aged mice.

Hood RL, Liguore WA, Moore C, Pflibsen L, Meshul CK
Brain research 2016 Sep 1;1646:535-542

Intervention with exercise restores motor deficits but not nigrostriatal loss in a progressive MPTP mouse model of Parkinson's disease.

Sconce MD, Churchill MJ, Greene RE, Meshul CK
Neuroscience 2015 Jul 23;299:156-74

Protective effects of cholecystokinin-8 on methamphetamine-induced behavioral changes and dopaminergic neurodegeneration in mice.

Gou H, Wen D, Ma C, Li M, Li Y, Zhang W, Liu L, Cong B
Behavioural brain research 2015 Apr 15;283:87-96

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Submitted by: Proteintech Group (provider)
Main image
Experimental details: The DAT antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human DAT. This antibody recognizes human, mouse, rat antigen. The DAT antibody has been validated for the following applications: ELISA, WB, IHC analysis.
Appendix: 22524-1-AP.pdf

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