MA5-24796

antibody from Invitrogen Antibodies

Targeting: SLC6A3 DAT, DAT1

Provider product page for MA5-24796

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [1]
Comments [0]
Validations
Western blot [3]
Immunocytochemistry [1]

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Product number: MA5-24796 - Provider product page
Provider: Invitrogen Antibodies
Product name: Dopamine Transporter Monoclonal Antibody (mAb16)
Antibody type: Monoclonal
Antigen: Synthetic peptide
Reactivity: Human, Mouse, Rat
Host: Mouse
Isotype: IgG
Antibody clone number: mAb16
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

SLC6A3 protein structure - MA5-24796 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Dopamine Transporter in rat striatal membrane tissue lysate. Sample was incubated in Dopamine Transporter monoclonal antibody (Product # MA5-24796).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Dopamine Transporter in rat striatal membrane tissue lysate. Sample was incubated in Dopamine Transporter monoclonal antibody (Product # MA5-24796).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using anti-Dopamine Transporter Monoclonal Antibody (mAb16) (Product # MA5-24796). A 68 kDa band corresponding to Dopamine Transporter (DAT) was observed across cell lines and brain tissues except Mouse, Rat Cerebellum and Mouse, Rat Heart which are reported to be low to negative expression models for Dopamine Transporter. Another band at 75 kDa was found only in the cell lines which could be another isoform of DAT. Uncharacterized bands at 260, 35, 15 kDa were observed in the samples tested. A band at 25 kDa was observed in mouse tissues which corresponds to circulating IgG in tissues. Membrane enriched extracts (30 µg) of BeWo (Lane1), MCF7 (Lane 2), SH-SY5Y (Lane 3), tissue extracts of Mouse Brain (Lane 4), Rat Brain (Lane 5), Mouse Cerebellum (Lane 6), Rat Cerebellum (Lane 7), Mouse Heart (Lane 8) and Rat Heart (Lane 9) were electrophoresed using Novex® NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).