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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- PA5-21382 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Creatine Kinase BB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: H1299, HeLaS3, mouse brain, rat brain. Predicted reactivity: Mouse (97%), Rat (97%), Xenopus laevis (88%), Pig (96%), Rabbit (96%), Chicken (97%), Rhesus Monkey (98%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.54 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Creatine Kinase BB Polyclonal Antibody (Product # PA5-21382). Sample (30 µg of whole cell lysate). A: H1299. B: HeLa S3. 12% SDS PAGE. Creatine Kinase BB Polyclonal Antibody (Product # PA5-21382) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Creatine kinase (brain) antibody detects Creatine kinase protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 10% SDS-PAGE, and blotted with Creatine kinase (brain) antibody Creatine Kinase BB Polyclonal Antibody (Product # PA5-21382) diluted by 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Creatine Kinase BB Polyclonal Antibody (Product # PA5-21382). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 10% SDS PAGE. Creatine Kinase BB Polyclonal Antibody (Product # PA5-21382) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Creatine Kinase BB in paraformaldehyde-fixed HeLa cells using a Creatine Kinase BB polyclonal antibody (Product # PA5-21382) at a 1:100 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Creatine Kinase BB antibody (Product # PA5-21382) at 1:100 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Creatine Kinase BB Polyclonal Antibody detects Creatine kinase (brain) protein at cytoplasm on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. Creatine Kinase BB Polyclonal Antibody (Product # PA5-21382) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded HSC3 xenograft, using Creatine kinase (brain) (Product # PA5-21382) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
