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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- PA5-27509 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Creatine Kinase BB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, H1299, HeLaS3, HepG2, NIH-3T3. Predicted reactivity: Mouse (95%), Rat (96%), Xenopus laevis (85%), Pig (98%), Rabbit (96%), Chicken (89%), Rhesus Monkey (99%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2.05 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Label-free proteomics uncovers energy metabolism and focal adhesion regulations responsive for endometrium receptivity.
Chen Q, Zhang A, Yu F, Gao J, Liu Y, Yu C, Zhou H, Xu C
Journal of proteome research 2015 Apr 3;14(4):1831-42
Journal of proteome research 2015 Apr 3;14(4):1831-42
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Creatine kinase (brain) using 30µg of H1299 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Creatine kinase (brain) polyclonal antibody (Product # PA5-27509) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Creatine kinase (brain) using 30 µg of NIH-3T3 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Creatine kinase (brain) polyclonal antibody (Product # PA5-27509) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Creatine Kinase BB Polyclonal Antibody (Product # PA5-27509). Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Creatine Kinase BB Polyclonal Antibody (Product # PA5-27509) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Creatine Kinase BB was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Creatine Kinase BB Polyclonal Antibody (Product # PA5 27509) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Creatine Kinase BB Polyclonal Antibody detects Creatine kinase (brain) protein at cytoplasm by immunofluorescent analysis. Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Creatine kinase (brain) stained by Creatine Kinase BB Polyclonal Antibody (Product # PA5-27509) diluted at 1:1,000. Blue: Fluoroshield with DAPI .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Creatine Kinase BB Polyclonal Antibody detects Creatine kinase (brain) protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Creatine kinase (brain) stained by Creatine Kinase BB Polyclonal Antibody (Product # PA5-27509) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human hepatoma, using Creatine kinase (brain) antibody (Product # PA5-27509) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
