GTX107155

antibody from GeneTex

Targeting: ZWINT KNTC2AP, SIP30, Zwint1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX107155

Provider

GeneTex

Proper citation

GeneTex Cat#GTX107155, RRID:AB_1952700

Product name

ZWINT antibody

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

References

Submitted references

Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2.

Zhou H, Wang T, Zheng T, Teng J, Chen J
Nature communications 2016 Jan 8;7:10151

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Phosphorylation of the Ndc80 complex protein, HEC1, by Nek2 kinase modulates chromosome alignment and signaling of the spindle assembly checkpoint.

Wei R, Ngo B, Wu G, Lee WH
Molecular biology of the cell 2011 Oct;22(19):3584-94

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample(30 ug whole cell lysate)A:293TB:MOLT4 (GTX27912) C:Raji (GTX27908)12% SDS PAGEGTX107155 diluted at 1:1000

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed 293T, using ZWINT(GTX107155) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ZWINT antibody detects ZWINT protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ZWINT protein stained by ZWINT antibody (GTX107155) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.Blue: Hoechst 33342 staining.