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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-89313 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LSM4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MLPLSLLKTA QNHPMLVELK NGETYNGHLV SCDNWMNINL REVICTSRDG DKFWRMPECY IRGSTIKYLR IPDEIIDMVK EEVVAKGRGR GGLQQQKQQK GRGMGGAGRG VFGGRGRGGI PGTGRGQPEK KPGRQAGKQ; Positive Samples: HepG2, MCF-7, Jurkat; Cellular Location: Nucleus
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.17 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of LSM4 was performed in HeLa cells using LSM4 Polyclonal Antibody (Product # PA5-89313).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of LSM4 in HeLa cells. Samples were incubated with LSM4 Polyclonal antibody (Product # PA5-89313) using a dilution of 0.0763888888888889. Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of LSM4 in 200 μg extracts of Jurkat cells. Samples were precipitated with 1 μg LSM4 Polyclonal antibody (Product # PA5-89313). Western blot was performed from the immunoprecipitate using LSM4 Polyclonal antibody (Product # PA5-89313) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of LSM4 in paraffin-embedded human colon. Samples were incubated with LSM4 Polyclonal antibody (Product # PA5-89313) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of LSM4 in paraffin-embedded rat testis. Samples were incubated with LSM4 Polyclonal antibody (Product # PA5-89313) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of LSM4 was performed in 200 µg extracts of Jurkat cells using LSM4 Polyclonal Antibody (Product # PA5-89313). Western blot was performed from the immunoprecipitate using LSM4 Polyclonal Antibody.
