Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-21629 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IPMK Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Raji, mouse brain. Predicted reactivity: Mouse (91%), Rat (90%), Bovine (92%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.34 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IPMK using 30 µg of H1299 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an IPMK polyclonal antibody (Product # PA5-21629) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using IPMK Polyclonal Antibody (Product # PA5-21629). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 10% SDS PAGE. IPMK Polyclonal Antibody (Product # PA5-21629) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using IPMK Polyclonal Antibody (Product # PA5-21629). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with IPMK Polyclonal Antibody (Product # PA5-21629) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using IPMK Polyclonal Antibody (Product # PA5-21629). Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with IPMK Polyclonal Antibody (Product # PA5-21629) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of IPMK in paraformaldehyde-fixed HeLa cells using an IPMK polyclonal antibody (Product # PA5-21629) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using IPMK antibody (Product # PA5-21629) at 1:200 dilution.