PA5-26879

antibody from Invitrogen Antibodies

Targeting: GTSE1 B99, GTSE-1

Western blot

Immunohistochemistry

Antibody data

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Validation data

Product number: PA5-26879 - Provider product page
Provider: Invitrogen Antibodies
Product name: GTSE1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: This antibody is predicted to react with mouse based on sequence homology.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 400 µL
Concentration: 0.27 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

GTSE1 protein structure - PA5-26879 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: 1 FIGURE GTSE1 expression was elevated in cervical cancer tissues. (a) The mRNA levels of GTSE1 in 41 cervical cancer tissues and 30 normal cervical tissues were tested by RT-PCR (** p < 0.01). (b) IHC staining was carried out to assess the protein levels of GTSE1 in three cervical cancer tissues and three normal cervical tissues

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: 2 FIGURE Downregulation of GTSE1 impaired cell viability and migration in cervical cancer. (a) The protein levels of GTSE1 in SiHa, HeLa, CaSki, and C33A cells were determined by using western blotting assay. (b) The knockdown efficiency of shGTSE1 in SiHa and HeLa cells were determined by western blotting. (c) Colony formation assay was carried out for cell viability detection following cell infection with shNC or shGTSE1 in SiHa and HeLa cells. (d) CCK-8 assay was carried out to assess cell growth following cell infection with shNC or shGTSE1 in SiHa and HeLa cells. (e) Wound-healing assay was carried out for cell migration detection following cell infection with shNC or shGTSE1 in SiHa and HeLa cells. ( n = 3, p values were indicated in the figures)

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: 5 FIGURE Downregulation of GTSE1 inhibited in vivo tumor formation in cervical cancer cells. (a, b) Tumor size and volume induced by SiHa and HeLa cells. (c) The mRNA levels of LDHA in 41 cervical cancer tissues and 30 normal cervical tissues were tested by RT-PCR. (d, e) Western blotting and IHC assay were used to detect the expression of GTSE1, LDHA, and Ki67 in mice tumor tissues. The original western blot figures were shown in Supplementary File. ( n = 3, p values were indicated in the figures)