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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
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- Product number
- GTX101535 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX101535, RRID:AB_2037048
- Product name
- GAP43 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: IMR32 12% SDS PAGE GTX101535 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GAP43 antibody detects GAP43 protein by Western blot analysis.A. 50 ?g mouse brain lysate/extract10 % SDS-PAGEGAP43 antibody (GTX101535) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GAP43 antibody detects GAP43 protein by Western blot analysis.A. 50 ?g rat brain lysate/extract10 % SDS-PAGEGAP43 antibody (GTX101535) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Mouse tissue extract (50 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with GAP43 antibody (GTX101535) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rat tissue extract (50 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with GAP43 antibody (GTX101535) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GAP43 antibody detects GAP43 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: GAP43 protein stained by GAP43 antibody (GTX101535) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
