AP32844PU-N

antibody from Acris Antibodies GmbH

Targeting: GAP43 B-50, GAP-43, PP46

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

AP32844PU-N

Provider

Acris Antibodies GmbH

Product name

anti GAP43 (C-term)

Antibody type

Polyclonal

Antigen

Chickens were immunized with a synthetic peptide corresponding to the C-terminal region of Mouse GAP-43 coupled to keyhole limpet hemocyanin. Production: After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and the IgY concentration adjusted to 20 mg/ml. This preparation was then diluted 1/10 with PBS containing BSA as a carrier. Finally, the antibody preparation was filter-sterilized.

Reactivity

Mouse

Host

Chicken/Avian

Vial size

0.2 ml

Concentration

2.0 mg IgY/ml (based on Bradford assay readings using BSA as a standard).

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

GAP43 staining of cultured cortical neurons purified from the neonatal Mouse brain (green satining). Red staining corresponds to a Rabbit anti- Alpha III Spectrin. Blue staining corresponds to the Hoechst nuclear satin.