NB300-179

antibody from Novus Biologicals

Targeting: GAP43 B-50, GAP-43, PP46

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

NB300-179

Provider

Novus Biologicals

Proper citation

Novus Cat#NB300-179, RRID:AB_10002938

Product name

Rabbit Polyclonal GAP-43 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specific for the ~50k Gap-43 protein phosphorylated at Ser41. In some tissues the antibody also recognizes a higher molecular weight protein that is also recognized by the pan Gap-43 antibody, that may be a Gap-43 aggregate or oligomer. Immunolableing is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Immunolabeling is completely eliminated by treatment with gamma-Ptase.

Reactivity

Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: GAP43 [p Ser41] Antibody [NB300-179] - Western blot of rat cortex lysate showing specific immunolabeling of the ~50k Gap-43 protein phosphorylated at Ser41 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-phosphatase (1200 units for 30 min) before being exposed to the GAP-43 Ser41 antibody. The immunolabeling of GAP-43 is completely eliminated by treatment with lambda-phosphatase.