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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- RQ5949 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- GAP43 Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific GAP43 antibody is suitable for use in Western blot/Flow cytometry/Immunohistochemistry/Immunofluorescence applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the GAP43 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat brain, 2) mouse brain, 3) human U-87 MG and 4) mouse Neuro-2a lysate with GAP43 antibody. Predicted molecular weight ~43 kDa with possible 38 kDa band (Ref 1).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE rat brain with GAP43 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE rat brain with GAP43 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human HepG2 cells with GAP43 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GAP43 antibody.
