- PA1-16729 - Invitrogen Antibodies GAP43 antibody

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of GAP43 in different tissue and cell lysates. Samples were incubated in GAP43 polyclonal antibody (Product # PA1-16729 using a dilution of 1:20,000. Antibody in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] SH-SY5Y cells, [7] C6 cells. Single band at 43 kDa mark corresponds to GAP43 protein. The GAP43 protein is detected only in the lysates of neuronal origin. C6 cells are a rat glioma cell line and do not express GAP43 protein.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of GAP43 in homogenate of cow cerebellum. Samples were incubated in GAP43 polyclonal antibody (Product # PA1-16729). A prominent band running at ~43 kDa represents the full length GAP-43.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot was performed using Anti-GAP43 Polyclonal Antibody (Product # PA1-16729) and a 38-48kDa band corresponding to GAP43 was observed across cell lines and tissues tested except in A549, A-431 and Mouse Lung which are reported to be low to negative for GAP43 expression. An increase in GAP43 expression was observed in SH-SY5Y on neuronal differentiation. Membrane enriched extracts (30 µg lysate) of SH-SY5Y (Lane 1), SH-SY5Y differentiated to neurons (Lane 2), SK-N-SH (Lane 3), IMR-32 (Lane 4), A549 (Lane 5), A-431 (Lane 6), Mouse Lung (Lane 7), Mouse Brain (Lane 8) and Rat Brain (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1ug/ml) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunofluorescence of GAP-43 (green), a molecular marker of neurite outgrowth, demonstrates intense staining in overexpressing wild-type PS-1 (E) PC-12 cells. (Teo, et al, 2005)

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunocytochemistry analysis of GAP43 in Cortical neuron-glial cell culture from E20 rat. Samples were incubated in GAP43 polyclonal antibody (Product # PA1-16729) using a dilution of 1:2000. This antibody in green, and costained with mouse mAb to vimentin, dilution 1:2,000, in red. Blue: DAPI staining of nuclear DNA. GAP43 antibody labels protein expressed in the axonal membrane of neuronal cells, while vimentin antibody stains intermediate filaments in fibroblasts and other non-neuronal cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunocytochemistry analysis of GAP43 in Rat E18 mixed neuron/glia cultures. Samples were incubated in GAP43 polyclonal antibody (Product # PA1-16729). GAP43 (red) and 5B10, mouse monoclonal to MAP-tau (green).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry analysis of GAP43 in mixed neuron/glial cultures. Samples were incubated in GAP43 polyclonal antibody (Product # PA1-16729). This antibody (red), blue is DNA staining.

PA1-16729