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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [6]
- Immunocytochemistry [1]
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- Product number
- PA5-27448 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GAP43 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: IMR32, mouse hippocampus, rat hippocampus.
- Concentration
- 0.55 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GAP43 using 50 µg mouse brain lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a GAP43 polyclonal antibody (Product # PA5-27448) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GAP43 using 50 µg rat brain lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a GAP43 polyclonal antibody (Product # PA5-27448) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GAP43 was performed by separating 50 µg of mouse tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a GAP43 Polyclonal Antibody (Product # PA5-27448) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using GAP43 Polyclonal Antibody (Product # PA5-27448). Sample (30 µg of whole cell lysate). Lane A: IMR32. 12% SDS PAGE. GAP43 Polyclonal Antibody (Product # PA5-27448) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GAP43 was performed by separating 50 µg of rat tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a GAP43 Polyclonal Antibody (Product # PA5-27448) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti- GAP43 Polyclonal Antibody (Product # PA5-27448 ) and bands around 38-48 kDa corresponding to GAP43 was observed in Mouse and Rat Brain but not in other tissues tested, which are reported to be negative for GAP43 expression. Additional faint uncharacterized band (*) was observed in Rat Brain. Tissues extracts (20 µg lysate) of Mouse Lung(Lane 1), Rat Lung (Lane 2), Mouse Kidney (Lane 3), Rat Kidney (Lane 4), Mouse Brain (Lane 5) and Rat Brain (Lane 6) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 0.25 µg/mL, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of GAP43 was performed in DIV9 rat E18 primary cortical neurons fixed in 4% paraformaldehyde at RT for 15 min. Green: GAP43 Polyclonal Antibody (Product # PA5-27448) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.
